Study of Rotary Cell Culture System-Induced Microgravity Effects on Cancer Biomarkers

Since humans started to explore the possibilities of life beyond earth, space missions began unmanned in the beginning and late with astronauts to explore the curiosity. There are several factors in space which are different from that of the earth's atmosphere, including gravity, cosmic radiation, extreme temperatures, and heavy ions. The space environment consists of very less gravitational force, which is often termed as microgravity. Microgravity, along with radiation in space, is known to suppress the immune system of astronauts, and hence, the chances of getting diagnosed with cancer could not be overlooked. Microgravity simulators are used to generate microgravity in laboratory conditions, and it could be a very beneficial tool to study the effect of altered gravity on various types of cells, including cancer cells. Cancer cells produce several biomarkers which are used for cancer diagnosis and prognosis. Protein biomarkers are indispensable for the ongoing fight against cancer. In this chapter, we describe the culture of both anchorage-dependent and suspension cell lines using rotary cell culture system (RCCS) to induce microgravity condition and its effect on protein biomarkers.