Related Experiment Video

Updated: Oct 6, 2025

A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells
05:36

A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells

Published on: September 6, 2018

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Expression and regulation of estrogen receptor 2 and its coregulators in mouse granulosa cells

Chihiro Emori1,2, Takuya Kanke1, Haruka Ito1

  • 1Laboratory of Applied Genetics, Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.

The Journal of Reproduction and Development
|January 20, 2022
PubMed

Insights

Oocyte-derived factors regulate estrogen signaling in mouse ovarian granulosa cells. Oocytes control estrogen receptor 2 and coregulator expression, crucial for follicle development.

Area of Science:

  • Reproductive Biology
  • Endocrinology
  • Cell Biology

Background:

  • Estrogen and oocyte-derived paracrine factors (ODPFs) are vital for ovarian follicle development.
  • The interplay between these factors and their regulatory mechanisms remain incompletely understood.

Purpose of the Study:

  • To investigate if ODPFs influence estrogen signaling by modulating estrogen receptor (ESR) and coregulator expression in mouse granulosa cells.
  • To elucidate the role of ODPFs in the cooperative signaling between oocytes and granulosa cells.

Main Methods:

  • Differential expression analysis of ESR coregulator transcripts in cumulus versus mural granulosa cells (MGCs).
  • Treatment of cumulus-oocyte complexes with ODPFs and SB431542 (transforming growth factor beta receptor I inhibitor).
  • Quantification of ESR2 protein and transcript levels in different granulosa cell types.

Main Results:

  • ODPFs significantly suppressed specific ESR coregulator transcripts in cumulus cells, an effect reversed by SB431542.
  • Mural granulosa cells displayed higher ESR2 protein and transcript levels compared to cumulus cells.
  • ODPFs enhanced Esr2 expression in cumulus cells but not in MGCs.

Conclusions:

  • Oocyte-derived factors regulate ESR2 and its coregulator expression in cumulus cells.
  • This regulation represents a key mechanism in estrogen-oocyte cooperation during antral follicle development in mice.
Keywords:
Cumulus cellsEstrogen receptor 2Oocytes

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