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Related Experiment Video

Updated: Oct 6, 2025

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System
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Highly Sensitive and Specific SARS-CoV-2 Serological Assay Using a Magnetic Modulation Biosensing System.

Shira Avivi-Mintz1, Yaniv Lustig2,3, Victoria Indenbaum2

  • 1Faculty of Engineering, The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Max and Anna Webb Street, Ramat Gan 5290002, Israel.

Biosensors
|January 20, 2022
PubMed
Summary

A new Magnetic Modulation Biosensing assay offers a highly sensitive and rapid method for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) IgG antibodies, crucial for tracking infections and immunity.

Keywords:
COVID-19SARS-CoV-2fluorescence-based assaymagnetic modulationoptical biosensingserology

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Area of Science:

  • Immunology and Infectious Diseases
  • Biotechnology and Biosensing

Background:

  • Accurate serological assays are vital for understanding viral infections, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
  • Detection of anti-SARS-CoV-2 IgG antibodies is important for assessing immunity and facilitating international travel.

Purpose of the Study:

  • To develop and evaluate a novel Magnetic Modulation Biosensing (MMB) system for sensitive and specific detection of anti-SARS-CoV-2 IgG antibodies.
  • To compare the performance of the MMB-based assay against the traditional enzyme-linked immunosorbent assay (ELISA).

Main Methods:

  • Utilized a Magnetic Modulation Biosensing (MMB) system combined with the receptor-binding domain of the SARS-CoV-2 spike protein.
  • Tested the assay using known anti-SARS-CoV-2 IgG antibodies, RT-qPCR confirmed SARS-CoV-2 patient samples, healthy controls, and vaccinee samples.
  • Compared analytical sensitivity (limit of detection) and clinical performance (sensitivity, specificity, turnaround time) with ELISA.

Main Results:

  • The MMB-based assay exhibited a significantly lower limit of detection (~6-fold) compared to ELISA (129 ng/L vs. 817 ng/L).
  • MMB detected earlier increases in IgG concentration post-vaccination.
  • Clinical performance was comparable to ELISA (93% sensitivity, 98% specificity for MMB vs. 92% sensitivity, 99% specificity for ELISA) but with a substantially faster turnaround time (45 min vs. 245 min).

Conclusions:

  • The novel MMB-based assay provides a highly sensitive and specific method for detecting anti-SARS-CoV-2 IgG antibodies.
  • Its superior analytical sensitivity, rapid detection, and simplicity make it a promising alternative to ELISA for antibody testing.
  • This assay could be valuable for monitoring viral infection status and immune response.