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Related Experiment Video

Updated: Oct 6, 2025

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

Published on: August 7, 2021

High-speed fluorescence image-enabled cell sorting.

Daniel Schraivogel¹, Terra M Kuhn², Benedikt Rauscher¹

¹Genome Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.

Science (New York, N.Y.)

|January 20, 2022

Summary

This summary is machine-generated.

High-speed image-enabled cell sorting (ICS) precisely isolates single cells based on morphology and protein localization. This advanced cell sorting technology accelerates genome-wide screening and expands phenotypic analysis capabilities.

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Last Updated: Oct 6, 2025

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

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Published on: August 7, 2021

Fluorescence Activated Cell Sorting of Plant Protoplasts

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Published on: February 18, 2010

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting FACS

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting FACS

Published on: July 10, 2010

Area of Science:

Cell Biology
Biotechnology
Genomics

Background:

Isolating single cells with specific spatial and morphological traits is a significant technical hurdle.
Current cell sorting methods lack the resolution to capture complex cellular phenotypes.

Purpose of the Study:

To develop a high-speed image-enabled cell sorting (ICS) method for precise single-cell isolation.
To expand the capabilities of cell sorting and pooled genetic screening through advanced phenotypic analysis.

Main Methods:

Established high-speed image-enabled cell sorting (ICS) capable of recording multicolor fluorescence images and sorting cells at 15,000 events/sec.
Utilized ICS to quantify cell morphology, protein localization, and mitotic stages for enhanced cell cycle analysis.
Combined ICS with CRISPR-pooled screens for rapid genome-wide screening.

Main Results:

ICS successfully quantifies cell morphology and protein localization, improving resolution in cell cycle analyses.
Enabled completion of genome-wide image-based screens in approximately 9 hours.
Demonstrated identification of nuclear factor κB (NF-κB) pathway regulators.

Conclusions:

ICS significantly broadens the phenotypic space accessible for cell sorting and pooled genetic screening.
This technology offers a powerful tool for analyzing complex cellular phenotypes and accelerating biological discovery.