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Measuring hyperemic response to light flicker stimulus using continuous laser speckle flowgraphy in mice
Michelle R Tamplin1, Kimberly A Broadhurst2, Anthony H Vitale2
1Department of Radiation Oncology, University of Iowa, Iowa City, IA, USA.
Experimental Eye Research
|January 20, 2022
Summary
We developed a new method using laser speckle flowgraphy to measure light-induced blood flow changes in mouse retinas. This reliable technique accurately quantifies neurovascular coupling, aiding research into vascular disorders.
Area of Science:
- Ophthalmology
- Neuroscience
- Vascular Biology
Background:
- Neurovascular coupling alterations are linked to ocular, cerebral, and systemic vascular diseases.
- Dynamic vessel analysis measures neurovascular coupling in the eye using light flicker stimuli.
Purpose of the Study:
- To present a novel protocol for quantifying light-flicker-induced hyperemia in the C57/Bl6J mouse retina.
- To adapt human-based protocols for mouse models using laser speckle flowgraphy (LSFG).
Main Methods:
- Developed and validated a new protocol for light-flicker stimulation in mouse retinas.
- Utilized laser speckle flowgraphy (LSFG) to acquire continuous time-series data of retinal blood flow.
- Adapted protocols from human studies for application in a mouse model.
Main Results:
- Successfully quantified light-flicker induced hyperemia in the mouse retina.
- Detected a significant increase in retinal blood flow following the light stimulus.
- Demonstrated low variability in responses across multiple imaging sessions.
Conclusions:
- The developed LSFG protocol provides a reliable method for assessing light-induced hyperemia in mouse retinas.
- This technique enables serial studies of neurovascular coupling in mouse models.
- The findings support the use of this protocol in investigating ocular and systemic vascular disorders.
