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A Rapid Method for Postmortem Vitreous Chemistry-Deadside Analysis.

Brita Zilg¹, Kanar Alkass¹, Robert Kronstrand²

¹Forensic Research Laboratory, Department of Oncology-Pathology, Karolinska Institute, 171 77 Stockholm, Sweden.

|January 21, 2022

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Analyzing postmortem vitreous fluid for electrolytes and glucose using blood gas instruments provides rapid, reliable results without sample pretreatment. This method simplifies early death investigation and autopsy planning.

Area of Science:

Forensic Science
Clinical Chemistry
Postmortem Toxicology

Background:

Vitreous fluid analysis is crucial in forensic investigations for toxicology and estimating time since death.
Early results for electrolytes (potassium, sodium, chloride) and glucose aid police and autopsy decisions.
Standard analysis methods can be time-consuming and require sample pretreatment.

Purpose of the Study:

To evaluate the impact of different sampling and pre-analytical treatments on vitreous fluid analysis.
To assess the suitability of blood gas instruments for rapid postmortem vitreous analysis.
To compare results from blood gas instruments with conventional multi-analysis instruments.

Main Methods:

Vitreous fluid samples were collected and analyzed using blood gas instruments.

Keywords:

electrolytes forensic medicine glucose postmortem vitreous

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Different sampling sites (eyes, center, whole) and pre-analytical treatments were tested.

Samples were also analyzed using a standard multi-analysis instrument for comparison.

Main Results:

Consistent results were obtained regardless of sampling site (right/left eye, center, whole vitreous).
Blood gas instruments demonstrated very low imprecision, with no need for centrifugation or dilution.
Conventional instruments required dilution for high-viscosity samples, potentially affecting accuracy.

Conclusions:

Blood gas instruments offer a rapid and reliable method for analyzing postmortem vitreous fluid for electrolytes and glucose.
Eliminating sample pretreatment simplifies the analysis process and speeds up results.
This approach enhances the efficiency of early postmortem investigations.