Related Experiment Video
Updated: Oct 6, 2025
09:43
Isolation, Culture, and Characterization of Prostate Cancer-Associated Fibroblasts
Published on: August 1, 2025
269
Increased Expression and Altered Cellular Localization of Fibroblast Growth Factor Receptor-Like 1 (FGFRL1) Are
Lan Yu1, Mervi Toriseva1, Syeda Afshan1
1Institute of Biomedicine and FICAN West Cancer Centre, University of Turku and Turku University Hospital, 20520 Turku, Finland.
Cancers
|January 21, 2022
Summary
Fibroblast growth factor receptor-like 1 (FGFRL1) is upregulated in prostate cancer (PCa). Nuclear FGFRL1 predicts recurrence, while FGFRL1 knockdown suppresses tumor growth, suggesting its role in PCa progression.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- Fibroblast growth factor receptors (FGFRs) 1-4 are implicated in prostate cancer (PCa) regulation.
- The specific role of FGFR-like 1 (FGFRL1) in PCa pathogenesis remains largely undefined.
Purpose of the Study:
- To investigate the expression and functional role of FGFRL1 in prostate cancer.
- To determine if FGFRL1 can serve as a prognostic biomarker for PCa patients.
Main Methods:
- Quantitative reverse transcription PCR (qRT-PCR) and immunohistochemistry on patient tissue microarrays (TMAs).
- In vitro studies involving FGFRL1 knockdown (KD) in PC3M cells and mouse xenograft models.
- Cox regression analysis for prognostic marker evaluation and transcriptomic analysis.
Main Results:
- FGFRL1 expression is significantly upregulated in PCa tissues.
- Membranous FGFRL1 negatively correlates with Gleason scores (GSs) and Ki67, while cytoplasmic and nuclear FGFRL1 correlate positively.
- Nuclear FGFRL1 independently predicts biochemical recurrence post-prostatectomy; FGFRL1-KD suppresses tumor growth and alters gene expression related to differentiation and tumor-stromal interactions.
Conclusions:
- FGFRL1 upregulation and altered subcellular localization contribute to PCa progression.
- Nuclear FGFRL1 shows potential as a prognostic biomarker for PCa recurrence.
- FGFRL1 acts as a decoy receptor, and its modulation impacts FGFR signaling and tumor growth.
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