Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Centrioles and Centrosomes01:13

Centrioles and Centrosomes

3.9K
Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or...
3.9K
Centrosome Duplication02:25

Centrosome Duplication

4.2K
The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
4.2K
Spindle Assembly02:50

Spindle Assembly

3.8K
Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a...
3.8K
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

4.6K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
4.6K
Attachment of Sister Chromatids02:57

Attachment of Sister Chromatids

3.5K
As cells progress into mitosis, the nuclear envelope breaks down, and the condensed chromosomes are exposed to the array of bipolar microtubules of the mitotic spindle. The kinetochore, a large, disc-shaped protein complex, is present at the centromere region of the sister chromatids and acts as a binding site for the microtubules.  Usually, the plus-end of a single microtubule is embedded within the kinetochore. However, some kinetochores first establish lateral contact with the side-wall...
3.5K
The Mitotic Spindle02:27

The Mitotic Spindle

6.9K
The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures...
6.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Status Epilepticus Alters the Function of Brain-Derived Extracellular Vesicles.

Journal of extracellular vesicles·2026
Same author

Nicotinamide N-methyltransferase as a therapeutic target in taxane-resistant castration-resistant prostate cancer.

Cell death discovery·2026
Same author

Identification of epigenetic monotherapy candidates in taxane-resistant CRPC.

Turkish journal of biology = Turk biyoloji dergisi·2026
Same author

Nanoparticle-encapsulated neuropeptide Y provides robust seizure protection in SCN1A-derived epilepsy.

Epilepsia·2025
Same author

RESTRICT-seq enables time-gated CRISPR screens and uncovers novel epigenetic dependencies of SCC resistance.

bioRxiv : the preprint server for biology·2025
Same author

Epidrug screening identifies type I PRMT inhibitors as modulators of lysosomal exocytosis and drug sensitivity in cancers.

Cell death & disease·2025

Related Experiment Video

Updated: Oct 6, 2025

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
07:14

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

8.4K

Keep Calm and Carry on with Extra Centrosomes.

Batuhan Mert Kalkan1, Selahattin Can Ozcan2, Nicholas J Quintyne3

  • 1Graduate School of Health Sciences, Koç University, Istanbul 34450, Turkey.

Cancers
|January 21, 2022
PubMed
Summary

Cancer cells often have extra or abnormal centrosomes, which are key drivers of tumor growth and genetic instability. This review explores how cancer cells amplify centrosomes and survive these dangerous anomalies, focusing on centrosome clustering.

Keywords:
cancercentrosomechromosomal instabilityclusteringmultipolar spindles

More Related Videos

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

15.5K
Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

15.3K

Related Experiment Videos

Last Updated: Oct 6, 2025

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
07:14

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

8.4K
Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

15.5K
Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

15.3K

Area of Science:

  • Cell Biology
  • Cancer Biology
  • Genetics

Background:

  • Centrosome number and structure aberrations are hallmarks of cancer.
  • Centrosome anomalies are linked to chromosome instability and tumor progression.
  • The link between centrosome abnormalities and cancer has been studied for over a century.

Purpose of the Study:

  • To review mechanisms of cancer cell centrosome amplification.
  • To explore how cancer cells tolerate centrosome anomalies.
  • To focus on the role of centrosomal clustering in cancer.

Main Methods:

  • Literature review of recent advances in cancer research.
  • Focus on mechanisms of centrosome amplification in cancer.
  • Examination of cancer cell tolerance strategies for centrosome anomalies.

Main Results:

  • Cancer cells amplify centrosomes through various mechanisms.
  • Cancer cells develop strategies to cope with centrosome amplification.
  • Centrosomal clustering is a key mechanism for tolerating supernumerary centrosomes.

Conclusions:

  • Centrosome amplification is a critical event in cancer development.
  • Cancer cells employ specific mechanisms to manage centrosome abnormalities.
  • Understanding these mechanisms, particularly centrosomal clustering, offers insights into cancer progression and potential therapeutic targets.