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A rapid enhanced chemiluminescent immunoassay for allergen-specific IgE antibodies
H J Stiles1, H J Bicknell, J A Matthews
1Wolfson Research Laboratories, Department of Clinical Chemistry, Queen Elizabeth Medical Centre, Birmingham, UK.
Journal of Bioluminescence and Chemiluminescence
|September 1, 1986
Abstract:
An enhanced chemiluminescent immunoassay is described for the measurement of allergen-specific IgE antibodies. The assay is demonstrated for pollen from four grass species. A comparison was made between results obtained by this method and those obtained by the radioallergosorbent (RAST) procedure; a high degree of correlation (r = 0.95) was found for D. glomerata specific IgE. The assay is rapid and can be carried out in under 1 hour. The advantages of the luminescent assay as compared with the RAST procedure are discussed.