Analysis Comparison for Rapid Identification of Pathogenic Virus from Infected Tissue Samples

Area of Science:

• Virology
• Molecular Biology
• Genomics

Background:

• Rapid identification of infectious agents is critical for timely diagnosis and treatment.
• Previous studies utilized the rapid determination of viral genome sequences (RDV) method for swab samples.

Purpose of the Study:

• To investigate suitable methods for rapid pathogen identification from infected tissue samples.
• To compare the efficacy of the RDV method and next-generation sequencing (NGS) for influenza virus detection in mouse lung tissue.

Main Methods:

• Influenza virus was inoculated into mice to generate infected lung tissue samples.
• RNA was extracted, converted to cDNA, and analyzed using the RDV method and NGS (MiSeq and MinION).

Main Results:

• The RDV method failed to detect the influenza virus in infected tissue samples.
• NGS successfully detected influenza virus reads in the same samples.
• MinION sequencing demonstrated shorter library and sequence preparation times compared to MiSeq.

Conclusions:

• NGS is a more suitable method than the RDV method for rapid viral pathogen identification in infected tissues.
• MinION sequencing is currently the preferred method for rapid viral identification from infectious samples due to its speed.