Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Solvating Effects02:12

Solvating Effects

8.0K
An understanding of the solvating effect helps rationalize the relation between solvation and acidity of the compound. In addition, this also explains the relative stability of conjugate bases for compounds with different pKa values. This lesson details, in-depth, the principle of solvating effects. The strength of an acid and the stability of its corresponding conjugate base are determined using pKa values. This observed relationship is a consequence of solvation, which is the interaction...
8.0K
Introduction to Mechanisms of Enzyme Catalysis01:13

Introduction to Mechanisms of Enzyme Catalysis

9.1K
For many years, scientists thought that enzyme-substrate binding took place in a simple "lock-and-key" fashion. This model stated that the enzyme and substrate fit together perfectly in one instantaneous step. However, current research supports a more refined view scientists call induced fit. The induced-fit model expands upon the lock-and-key model by describing a more dynamic interaction between enzyme and substrate. As the enzyme and substrate come together, their interaction causes...
9.1K
RNA Stability01:53

RNA Stability

34.1K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
34.1K
Leveling Effect01:29

Leveling Effect

1.0K
In acid-base chemistry, the leveling effect refers to the limitation imposed by the solvent on the strength of acids and bases in solution. When a base stronger than the solvent's conjugate base is used, it deprotonates the solvent until the base is entirely consumed, making it ineffective against weaker acids. Conversely, an acid stronger than the solvent's conjugate acid protonates the solvent until the acid is depleted, rendering it ineffective against weaker bases. Essentially, the...
1.0K
Keto–Enol Tautomerism: Mechanism01:14

Keto–Enol Tautomerism: Mechanism

6.3K
The keto and enol forms are known as tautomers and they constantly interconvert (or tautomerize) between the two forms under acid or base catalyzed conditions. Both the reactions involve the same steps—protonation and deprotonation— although in the reverse order.
6.3K
Protein Denaturation01:28

Protein Denaturation

6.2K
The function of proteins depends on their native three-dimensional structure, which is dictated by the amino acid sequence of the specific protein. Folding of the polypeptide chain takes place under specific conditions that energetically favor the folded conformation. In contrast, protein denaturation occurs spontaneously under unfavorable conditions that disrupt the integrity of the folded conformation. Thus, the chemical and physical environment of a protein, such as significant changes in pH...
6.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Allosteric Activation of GDH/TCA Pathway Reduces Pathological Build-Up and Promotes Neuronal Survival in an In Vitro Model of Alzheimer's Disease.

Biomolecules·2026
Same author

Structural basis of the lobster carapace blue colour mediated by an HPR protein.

bioRxiv : the preprint server for biology·2026
Same author

Novel Ti6Al4V Surface Treatment for Subperiosteal Dental Implants (Part II): Matrix Deposition and Osteogenic Markers.

Materials (Basel, Switzerland)·2026
Same author

Glyceraldehyde-induced metabolic defects in cortical astrocytes: implication for Alzheimer's disease pathogenesis and therapy.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Organelles storing Ca2+ in the brain cells: New druggable targets in neurodegenerative diseases.

Neural regeneration research·2026
Same author

A Hypersexuality Subset Behavior Induced by Aripiprazole Overdose in an Antipsychotic Naïve Patient: A Case Report and Review of the Literature.

Clinics and practice·2026
Same journal

Correction: Yalçın et al. Impact of SGLT2 Inhibitors on Cardiovascular Risk Scores, Metabolic Parameters, and Laboratory Profiles in Type 2 Diabetes. <i>Life</i> 2025, <i>15</i>, 722.

Life (Basel, Switzerland)·2026
Same journal

Correction: Schubert et al. Minimally Invasive Ablation Strategies for Renal Cell Carcinoma Patients Ineligible for Surgery. <i>Life</i> 2026, <i>16</i>, 73.

Life (Basel, Switzerland)·2026
Same journal

Blood Group Antigen Combinations and COVID-19: Complexity, Associations and Possible Clinical Relevance.

Life (Basel, Switzerland)·2026
Same journal

Beyond HPV in Eastern Europe: Genotype Distribution, Molecular Biomarkers, Vaginal Microbiome, and Implications for Cervical Cancer Prevention.

Life (Basel, Switzerland)·2026
Same journal

Therapeutic Effects of <i>Scutellaria baicalensis</i> Georgi Extract and Baicalein on Olfactory Dysfunction and Neurobehavioral Alterations in a Methimazole-Induced Injury Model.

Life (Basel, Switzerland)·2026
Same journal

The Effects of Unstable Strength Training on Lower Limb Stability in Adolescent Volleyball Players in China.

Life (Basel, Switzerland)·2026
See all related articles

Related Experiment Video

Updated: Oct 6, 2025

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project
07:22

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

Published on: February 11, 2019

28.5K

Taurine Stabilizing Effect on Lysozyme.

Leonardo Mastrella1, Paolo Moretti1, Silvia Pieraccini2

  • 1Department of Life and Environmental Sciences, Marche Polytechnic University, I-60131 Ancona, Italy.

Life (Basel, Switzerland)
|January 21, 2022
PubMed
Summary
This summary is machine-generated.

Taurine stabilizes proteins and alters amyloid formation, potentially impacting neurodegenerative disease therapies. This study investigates taurine

Keywords:
Circular DichroismSmall Angle X-ray Scatteringamyloidlysozymeprotein solvationspectroscopytaurine

More Related Videos

Synthesis of 1,2-Azaborines and the Preparation of Their Protein Complexes with T4 Lysozyme Mutants
08:56

Synthesis of 1,2-Azaborines and the Preparation of Their Protein Complexes with T4 Lysozyme Mutants

Published on: March 25, 2017

7.7K
PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
09:31

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase

Published on: September 26, 2020

4.5K

Related Experiment Videos

Last Updated: Oct 6, 2025

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project
07:22

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

Published on: February 11, 2019

28.5K
Synthesis of 1,2-Azaborines and the Preparation of Their Protein Complexes with T4 Lysozyme Mutants
08:56

Synthesis of 1,2-Azaborines and the Preparation of Their Protein Complexes with T4 Lysozyme Mutants

Published on: March 25, 2017

7.7K
PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
09:31

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase

Published on: September 26, 2020

4.5K

Area of Science:

  • Biochemistry
  • Biophysics
  • Neuroscience

Background:

  • Taurine is an organic osmolyte with anti-inflammatory and antioxidant properties.
  • Taurine shows therapeutic potential in neurodegenerative disorders, but its specific mechanisms are unclear.
  • The protein-stabilizing effects of taurine are known but not extensively studied.

Purpose of the Study:

  • To investigate taurine's stabilizing effect on lysozyme thermal denaturation.
  • To explore taurine's influence on lysozyme aggregation into amyloid fibrils.
  • To evaluate the cytotoxicity of taurine-modified amyloid fibrils.

Main Methods:

  • Circular Dichroism (CD) spectroscopy for protein thermal denaturation.
  • Small Angle X-ray Scattering (SAXS) for protein-protein interactions.
  • UV/Vis spectroscopy with Congo Red and Atomic Force Microscopy (AFM) for amyloid aggregation.
  • Cytotoxicity assays on SH-SY5Y neuroblastoma cells.

Main Results:

  • Taurine modifies protein-protein interactions in native lysozyme.
  • Taurine alters amyloid aggregation patterns without complete inhibition.
  • Amyloid fibrils formed with taurine exhibit different cytotoxicities.

Conclusions:

  • Taurine influences lysozyme's structural properties and aggregation behavior.
  • Taurine's effects on amyloid formation may have implications for neurodegenerative diseases.
  • Further research is needed to elucidate taurine's precise role in neuroprotection.