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Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

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Related Experiment Video

Updated: Jul 3, 2026

Bioassays for Monitoring Insecticide Resistance

Bioassays for Monitoring Insecticide Resistance

Published on: December 30, 2010

RNAi for Western Corn Rootworm Management: Lessons Learned, Challenges, and Future Directions.

Molly Darlington¹, Jordan D Reinders¹, Amit Sethi²

¹Department of Entomology, University of Nebraska, Lincoln, NE 68583, USA.

|January 21, 2022

Summary

This summary is machine-generated.

Western corn rootworm (WCR) management is challenging due to insecticide resistance. A new RNA interference (RNAi) maize targets the WCR snf7 gene, offering a novel pest control strategy.

Keywords:

Diabrotica virgifera virgifera RNAi insect resistance management plant-incorporated protectant pyramid strategy western corn rootworm

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Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing

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Using Flight Mills to Measure Flight Propensity and Performance of Western Corn Rootworm, Diabrotica virgifera virgifera LeConte

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Published on: October 29, 2019

Area of Science:

Agricultural Entomology
Molecular Biology
Pest Management

Background:

Western corn rootworm (WCR), *Diabrotica virgifera virgifera*, is a major maize pest in the U.S. Corn Belt, causing significant economic losses.
WCR has evolved resistance to conventional management strategies, necessitating the development of novel control methods.
RNA interference (RNAi) is a gene-silencing mechanism that has been explored for pest control.

Purpose of the Study:

To introduce a novel RNA interference (RNAi) strategy for managing the western corn rootworm (WCR) in maize.
To highlight the development and approval of the first *in planta* RNAi product for insect control.

Main Methods:

Maize engineered to express double-stranded hairpin RNA targeting the WCR *snf7* gene.
Utilizing RNA interference (RNAi) to trigger gene silencing and insect mortality.
Combining RNAi trait with *Bt* proteins (Cry3Bb1 and Cry34Ab1/Cry35Ab1) for enhanced durability.

Main Results:

The U.S. Environmental Protection Agency approved the first *in planta* RNAi product in June 2017.
This product targets the WCR *snf7* gene, inducing mortality through RNA interference.
Commercial introduction of this WCR management tool is planned for 2022.

Conclusions:

RNA interference (RNAi) in maize offers a promising new tool for managing the resistant western corn rootworm (WCR).
The combination of RNAi and *Bt* proteins aims to provide durable and effective pest control.
This represents a significant advancement in the development of insect-protected crops.