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Mass-Rearing and Molecular Studies in Tortricidae Pest Insects
Published on: March 25, 2022
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Basic Studies Aiming at Orius minutus (Hemiptera: Anthocoridae) Mass-Rearing
Hye-Jeong Jun1, Kyoung-Su Kim2, Eun-Hye Ham1
1Institute for Bioresources, Osangkinsect Co., Ltd., Guri 11921, Korea.
Insects
|January 21, 2022
Summary
This study optimized mass-rearing for the biological control agent Orius minutus using artificial substrates and diets. Cork sheets proved most cost-effective, reducing rearing costs by 70.5%.
Area of Science:
- Agricultural Entomology
- Biological Control
- Insect Rearing
Background:
- Enhancing the usability of Orius minutus as a biological control agent requires simplifying its mass-rearing process.
- Development of alternative diets and artificial oviposition substrates is crucial for cost-effective insect production.
Purpose of the Study:
- To evaluate the economic viability of artificial oviposition substrates for mass-rearing Orius minutus.
- To compare the efficacy of different artificial substrates and feeding treatments on egg production and hatch rates.
- To develop a cost-effective, plant-free mass-rearing model for Orius minutus.
Main Methods:
- Comparison of Orius minutus egg hatch rates on plant substrates versus artificial substrates (cork sheets, rubber).
- Evaluation of five feeding treatments, including brine shrimp and Ephestia cautella eggs, on egg production in the fifth generation.
- Calculation of cost reduction achieved by the developed plant-free mass-rearing model.
Main Results:
- Cork sheets were identified as the most cost-effective artificial oviposition substrate for Orius minutus mass-rearing.
- No significant difference in egg laying was observed between the group fed iron-coated brine shrimp and moth eggs (61.3 eggs) and the control group (67.4 eggs).
- The developed plant-free model reduced rearing costs by 70.5% compared to conventional methods.
Conclusions:
- Artificial oviposition substrates, particularly cork sheets, significantly improve the cost-effectiveness of Orius minutus mass-rearing.
- Dietary treatments did not significantly impact egg production, suggesting flexibility in feeding regimes.
- The plant-free mass-rearing model offers substantial economic benefits for biological control applications.

