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Solid Lipid Nanoparticles Administering Antioxidant Grape Seed-Derived Polyphenol Compounds: A Potential Application
Adriana Trapani1, María Ángeles Esteban2, Francesca Curci1
1Department of Pharmacy-Drug Sciences, University of Bari "Aldo Moro", Via Orabona 4, 70125 Bari, Italy.
Molecules (Basel, Switzerland)
|January 21, 2022
Summary
Solid lipid nanoparticles (SLN) effectively deliver antioxidant grape seed extract (GSE) to fish cells, enhancing cell viability and antioxidant gene expression. This formulation shows promise for aquaculture nutrition and fish health.
Area of Science:
- Aquaculture Nutrition
- Nanotechnology in Animal Health
- Fish Cell Biology
Background:
- Nutrient delivery, particularly antioxidants, to fish cells is a significant challenge in aquaculture.
- Grape seed extract (GSE) offers valuable antioxidant compounds.
- Solid lipid nanoparticles (SLN) are a potential delivery system for lipophilic compounds.
Purpose of the Study:
- To develop and characterize solid lipid nanoparticles (SLN) for delivering grape seed extract (GSE) to fish cells.
- To evaluate the impact of GSE-loaded SLN on fish cell viability and gene expression.
- To explore the potential of SLN-GSE as a nutritional supplement in aquaculture.
Main Methods:
- Formulation of SLN using Gelucire® 50/13 and Precirol® ATO5, loaded with GSE.
- Characterization of SLN particle size, zeta potential, and structural integrity using Raman spectroscopy and X-ray diffraction.
- In vitro assessment of GSE-SLN effects on gilthead seabream and European sea bass cell lines, including viability assays and gene expression analysis.
Main Results:
- SLN formulations exhibited particle sizes between 139-283 nm and positive zeta potentials (+25.6 to +43.4 mV).
- GSE incorporation altered the structural properties of the lipid nanocarriers.
- GSE-SLN treatment generally increased fish cell viability and upregulated antioxidant gene expression, while downregulating hsp70 and cytoskeleton-related genes.
Conclusions:
- SLN composed of Gelucire® 50/13 and Precirol® ATO5 are suitable carriers for GSE.
- GSE-loaded SLN can positively influence fish cell health by enhancing antioxidant defense mechanisms.
- This SLN-GSE platform offers a promising strategy for improving fish cell viability and antioxidant capacity in aquaculture.
