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Updated: Oct 6, 2025
Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
Published on: March 7, 2018
A fluorescent aptasensor for Pb2+ detection based on gold nanoflowers and RecJf exonuclease-induced signal
Ruike Liu1, Baoshan He1, Huali Jin1
1College of Food Science and Technology, Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Henan University of Technology, Zhengzhou, 450001, PR China.
Abstract:
A fluorescent aptasensor was constructed to detect lead ion (Pb2+). Complementary DNA with sulfhydryl group (SH-cDNA) were loaded on gold nanoflowers materials (Au NFs) through Au-S bonds. Then, based on complementary base pairing between FAM modified Pb2+ aptamer (FAM-Apt) and Au NFs/SH-cDNA system, an Au NFs/SH-cDNA/FAM-Apt aptasensor was constructed. The fluorescence of FAM was quenched by Au NFs due to the fluorescence resonance energy transfer (FRET). Upon addition of Pb2+ and RecJf exonuclease (RecJf Exo) for 1.5 h, the Apt changed to a G-quadruplex structure due to the high affinity between Pb2+ and its aptamer, which lead to a large recovery in the fluorescence intensity. Under the optimized conditions, the presented aptasensor revealed high selectivity toward Pb2+ in the concentration range of 0.5 nM-1 μM, with a limit of detection (LOD) of 0.285 nM. Besides, the designed aptasensor was successfully used to recognize Pb2+ with excellent selectivity, reproducibility and stability in tap water and tea, providing a potential platform for Pb2+ detection in actual samples.
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