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Related Experiment Videos

Lab-made 3D printed stoppers as high-throughput cell migration screening tool.

Silvina Acosta1, Lucía Canclini2, Carlos Galarraga3

  • 1Epigenetics and Genomic Instability Laboratory, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.

SLAS Technology
|January 21, 2022
PubMed
Summary

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Researchers developed an affordable, 3D-printed device for wound healing assays to study cell migration. This reproducible, high-throughput method offers an economical alternative for biological and pharmaceutical research.

Area of Science:

  • Cell Biology
  • Biomedical Engineering
  • Drug Discovery

Background:

  • Cell migration is crucial for organism development and implicated in diseases.
  • Wound healing assays are vital for studying cell migration in basic biology and drug development.
  • Existing methods like scratch tests lack reproducibility and high-throughput capacity, while commercial options are costly.

Purpose of the Study:

  • To develop an inexpensive, reproducible, and high-throughput lab-made wound healing assay device.
  • To create 3D-printed stoppers for use in 96-well plates for cell migration assessment.
  • To validate the device's efficacy in evaluating cell migration and proliferation.

Main Methods:

  • Designed and fabricated 3D-printed stoppers for 96-well plates.
Keywords:
3D-printingEdU proliferation assayHaCaT cell linecell migrationwound healing assay

Related Experiment Videos

  • Assessed HaCaT cell viability and proliferation using the developed stoppers.
  • Evaluated the reproducibility and high-throughput capability of the stopper-based wound healing assay.
  • Tested the device's ability to differentiate treatments, including serum variations.

    • Main Results:

    • The 3D-printed stoppers did not impact HaCaT cell viability.
    • The initial wound size created by the stoppers was highly reproducible on a high-throughput scale.
    • The assay effectively evaluated cell migration and differentiated between treatments with and without fetal bovine serum.
    • Cell proliferation was successfully assessed within the wound healing model.

    Conclusions:

    • A novel, cost-effective, 3D-printed stopper-based wound healing assay was successfully developed.
    • This method provides a reproducible and high-throughput alternative to existing cell migration assessment strategies.
    • The assay is suitable for both cell migration and proliferation studies, offering economic benefits for research and pharmaceutical development.