Histidinibacterium aquaticum sp. nov., Isolated from Salt-Field Sea Water

A Gram stain-negative, aerobic, motile, and rod-shaped bacterium designated 176SS1-4^T was isolated from the salt-field sea water. Strain 176SS1-4^T grew well at 25 °C on marine agar. The taxonomic affiliation of the novel isolate was identified using the polyphasic approach. By comparative 16S rRNA gene sequence, it could be shown that strain 176SS1-4^T branches within the family Rhodobacteraceae and class Alphaproteobacteria and is related to Histidinibacterium lentulum B17^T (96.0% sequence similarity). The digital DNA-DNA hybridization and average nucleotide identity between strain 176SS1-4^T and H. lentulum B17^T was 19.2% and 77%. The genome comprises of 3,905,029 bp with a G + C content of 67.7 mol. Ubiquinone 10 (Q-10) was the major respiratory quinone. The major fatty acids were summed feature 8 (comprising C_18:1 ω7c and/or C_18:1 ω6c), C_16:0, C_19:0 cyclo ω8c, C_18:0, and C1_8:1 ω6c 11-methyl and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylcholine. On the basis of the genotypic and phenotypic characteristics, strain 176SS1-4^T can be placed as a novel species within the genus Histidinibacterium; the name Histidinibacterium aquaticum sp. nov. has been proposed, with type strain 176SS1-4^T (= KACC 19891^T = LMG 31032^T).