A Lab-on-a-Chip Based Automatic Platform for Continuous Nitrites Sensing in Aquaculture

In aquaculture, the density of fish stock, use of feeding, and surrounding environmental conditions can easily result in an excessive concentration of harmful compounds that require continuous monitoring. Chemical sensors are available for most of these compounds, however, operative conditions and continuous monitoring in water make the development of sensors suitable for long and unattended deployments difficult. A possible solution is the development of engineered automatic labs where the uptake of sample and the contact with water is reduced and the use of a minimal quantity of reagents enables the implementation of reliable chemical assays. In this paper, a platform for automatic chemical assays is presented. The concept is demonstrated with the detection of nitrites based on the well-known colorimetric Griess reaction. The platform is centered around a lab-on-a-chip where reagents and water samples are mixed. The color of the reaction product is measured with low-cost optoelectronic components. Results show the feasibility of the approach with a minimum detectable concentration of about 0.1 mg/L which is below the tolerance level for aquaculture farms.