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Updated: Oct 5, 2025

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies
Published on: May 6, 2022
Personalized gene-diet study of rs2239670 gene variants and dietary patterns among obese adults
Mahdieh Khodarahmi1, Mahdi Niknam1, Mahdieh Abbasalizad Farhangi1
1Department of Community Nutrition, Tabriz University of Medical Sciences, Tabriz, Iran.
Objective:
The aim of the present study was to assess the interaction of major dietary patterns with Cocaine- and Amphetamine-Regulated Transcript (CART) prepropeptide gene rs2239670 variant in apparently healthy obese Iranians.
Methods:
A total of 288 apparently healthy obese participants aged 20-50 years were recruited in this cross-sectional study. Usual dietary intake was assessed using a 147-item semi-quantitative validated food frequency questionnaire. Genotyping of the CART gene rs2239670 polymorphism was conducted by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
Results:
The results showed that the majority of the participants had GG genotype (65.4%). Three dietary patterns were extracted: prudent dietary pattern (PDP), legume dietary pattern (LDP), and mixed dietary pattern (MDP). There were significant interactions between PDP and rs2239670 variant on fat free mass (FFM) (p Interaction = 0.039), basal metabolic rate (BMR) (p Interaction = 0.039). Also, significant interactions were observed between LDP and rs2239670 polymorphism on the diastolic blood pressure (DBP) (p Interaction = 0.026), waist to hip ratio (WHR) (p Interaction = 0.050), agouti-related protein (AgRP) (p Interaction = 0.012), and α-melanocyte stimulating hormone (α-MSH) (p Interaction = 0.006).
Conclusions:
The CART rs2239670 SNP interacts with dietary patterns to affect some of the cardio-metabolic risk factors.
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