Characteristics, expression profile, and function of non-specific lipid transfer proteins of Populus trichocarpa

Plant non-specific lipid transfer proteins (nsLTPs) are involved in various physiological processes. However, the characteristics and function of LTPs in Populus trichocarpa are unclear. Here, we report the functional properties of type IV, V, and VI P. trichocarpa nsLTPs (PtLTPs). The IV, V, and VI PtLTPs clustered in the same clade shared similar gene structures and motif and distributions. Also, collinearity analysis revealed 2 and 7 gene pairs have tandem duplication and segmental duplication events, respectively. The expression patterns of type IV, V, and VI PtLTPs differed among poplar tissues. We investigated the effects of various stresses on the Potri.010G100600, Potri.010G196300, and Potri.016G104300 (type V LTPs) mRNA levels, and type V LTPs can respond to multiple stresses. Potri.008G061800 was localized to the cell wall, extracellular space, and plasma membrane. Glutathione-S-transferase-Potri.008G061800 obtained by prokaryotic expression had weakly inhibited the growth of Septotis populiperda in vitro. Taken together, our data show that type IV, V, and VI PtLTPs may be thought as novel regulators of plant stresses. They could be considered an effective genetic resource for molecular breeding in poplar.