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Updated: Oct 5, 2025

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
Published on: May 21, 2020
Transcriptome profiling reveals major structural genes, transcription factors and biosynthetic pathways involved in
Zechariah Effah1, Lingling Li2, Junhong Xie2
1State Key Laboratory of Arid Land Crop Science, Lanzhou, China; College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China; CSIR-Plant Genetic Resources Research Institute (PGRRI), P. O. Box 7, Bunso, Ghana.
Abstract:
The present study was undertaken to profile transcriptional changes in flag leaves between anthesis and end of grain filling stages of rainfed spring wheat cultivar under varying nitrogen (N) application rates: 0 kg/ha (NN), 52.5 kg/ha (LN), and 210 kg/ha (HN). A total of 4485 and 4627 differentially expressed genes (DEGs) were detected in LN and HN, respectively. The differential application of N altered several pathways; including plant hormone signal transduction, mitogen-activated protein kinase signaling pathway-plant, photosynthesis, phenylpropanoid biosynthesis and ATP-binding cassette transporters. Jasmonic acid, abscisic acid, salicylic acid and brassinosteroid related genes promoted leaf senescence in NN or LN, whereas auxin, gibberellin acid and cytokinins genes inhibited leaf senescence in HN. Major transcription factors: auxin/indole-3-acetic acid (AUX/IAA), no apical meristem (NAC) and WRKY expressed higher in either HN or LN than NN. The DEGs, pathways and transcription factors provide valuable insight for manipulation of leaf senescence and N remobilization in wheat.
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