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Updated: Oct 5, 2025
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Fluorescent Visualization of Mango-tagged RNA in Polyacrylamide Gels via a Poststaining Method
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Isolation and functional analysis of two CONSTANS-like 1 genes from mango
Yi-Hang Guo1, Cong Luo1, Yuan Liu1
1State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, 530004, Guangxi, China.
Plant Physiology and Biochemistry : PPB
|January 22, 2022
Summary
Two mango genes, MiCOL1A and MiCOL1B, regulate flowering time and enhance drought tolerance in plants. These CONSTANS-LIKE1 (COL1) homologs are crucial for plant development and stress response.
Area of Science:
- Plant Molecular Biology
- Genetics
- Plant Physiology
Background:
- The CONSTANS-LIKE1 (COL1) gene is vital for regulating photoperiodic flowering in plants.
- Understanding COL1 homologs in fruit trees like mango is essential for crop improvement.
Purpose of the Study:
- To isolate and characterize COL1 homolog genes (MiCOL1A and MiCOL1B) from mango (Mangifera indica L.).
- To investigate the role of MiCOL1A and MiCOL1B in flowering regulation and stress response.
Main Methods:
- Gene isolation and sequence analysis of MiCOL1A and MiCOL1B.
- Expression analysis in different organs and under seasonal conditions.
- Localization and functional assays (yeast, Arabidopsis overexpression).
Main Results:
- MiCOL1A and MiCOL1B genes were identified, encoding proteins with a CCT domain, belonging to the CO/COL IV family.
- Differential expression was observed in vegetative and reproductive organs, with higher levels during flower induction.
- Overexpression in Arabidopsis led to delayed flowering and enhanced drought tolerance.
Conclusions:
- MiCOL1A and MiCOL1B are involved in regulating flowering time in mango.
- These genes also contribute to plant stress tolerance, specifically drought resistance.
- Mango COL1 homologs have dual roles in development and stress response.
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