Related Experiment Video
Updated: Oct 5, 2025
Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
In vitro oxidation of hispidin and gallic acid by horseradish peroxidase
Damian Tarasek1, Hubert Wojtasek1, Khedidja Benarous2
1Institute of Chemistry, Opole University, Opole, Poland.
Abstract:
Gallic acid and hispidin have been previously described by us as inhibitors of horseradish peroxidase (Benarous, K., Benali, F. Z., Bekhaoua, I. C., and Yousfi, M. Journal of Biomolecular Structure & Dynamics, (2021) 39(18), 7168-7180). However, additional experiments have demonstrated that under the applied assay conditions both compounds are rapidly oxidized by this enzyme. After oxidation, the components of the reaction mixture undergo complex reactions giving products with much weaker absorption at the detection wavelength. This was interpreted by us as enzyme inhibition, which, however, is only apparent. In fact, the activity of horseradish peroxidase is not affected by these compounds, which was demonstrated by measurements of hydrogen peroxide consumption.Communicated by Ramaswamy H. Sarma.
Related Concept Videos
02:37Oxidation of Alcohols
The process of oxidation in a chemical reaction is observed in any of the three forms:
01:17Oxidation of Phenols to Quinones
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox...
01:20Radical Autoxidation
02:21Oxidation of Alkenes: Syn Dihydroxylation with Potassium Permanganate
02:04Oxidation of Alkenes: Anti Dihydroxylation with Peroxy Acids
01:15Oxidations of Aldehydes and Ketones to Carboxylic Acids
Aldehydes readily undergo oxidation in strong oxidizing agents such as potassium permanganate and chromic acid. The oxidation can also be carried out using mild oxidizing agents such as silver oxide. In fact, aldehydes can be easily oxidized...