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Updated: Oct 5, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

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Published on: August 16, 2024

Programmable Biosensors Based on RNA-Guided CRISPR/Cas Endonuclease.

Xiaolong Liu¹, Mubashir Hussain¹, Jianguo Dai¹

¹Shenzhen Key Laboratory of Fermentation, Purification and Analysis, Shenzhen Polytechnic, Shenzhen, 518055, China.

Biological Procedures Online

|January 24, 2022

Summary

Clustered regularly interspaced short palindromic repeat and CRISPR-associated protein (CRISPR-Cas) systems are revolutionizing nucleic acid detection for infectious diseases. This review highlights CRISPR-Cas biosensors for rapid, point-of-care diagnostics of pathogens and tumors.

Keywords:

Biosensors CRISPR/Cas Diagnostic Nucleic acids Pathogen

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Area of Science:

Biotechnology
Molecular Biology
Infectious Disease Diagnostics

Background:

Infectious diseases pose significant public health and economic threats globally.
Traditional nucleic acid detection methods face limitations in speed and precision.
CRISPR-Cas systems, originally for genome engineering, offer programmable precision.

Purpose of the Study:

To review the application of CRISPR-Cas systems as biosensors for nucleic acid detection.
To explore the capabilities of CRISPR-Cas in identifying genetic mutations and microbial pathogens.
To discuss the advantages and challenges of developing CRISPR-Cas for point-of-care (POC) diagnostics.

Main Methods:

Review of current literature on CRISPR-Cas enzyme utility in biosensing.
Analysis of CRISPR-Cas mechanisms for recognition, signal amplification, and detection.
Evaluation of CRISPR-Cas technology for diagnostic applications.

Main Results:

CRISPR-Cas systems function as effective biosensors for accurate nucleic acid detection.
These systems enable rapid, multiplexed biomarker identification for tumors and pathogens.
CRISPR-Cas technology demonstrates potential for improved rational redesign in biosensing.

Conclusions:

CRISPR-Cas biosensors represent a significant advancement in diagnostic technology.
The review provides insights into the current status and future potential of CRISPR-Cas for rapid POC diagnostics.
Overcoming current hurdles is crucial for widespread adoption of CRISPR-Cas diagnostic tools.