Lrrcc1 and Ccdc61 are conserved effectors of multiciliated cell function
Aude Nommick1, Camille Boutin1, Olivier Rosnet1
1Aix Marseille Univ, CNRS, IBDM, Turing Center for Living Systems, 13009 Marseille, France.
Journal of Cell Science
|January 24, 2022
Summary
Two basal body proteins, Lrrcc1 and Ccdc61, are crucial for multiciliated cell function in vertebrates. Their depletion disrupts cilia organization, leading to impaired fluid flow and increased susceptibility to bacterial infection.
Area of Science:
- Cell Biology
- Developmental Biology
- Eukaryotic Microbiology
Background:
- Ciliated epithelia are vital for locomotion and clearance in diverse organisms.
- Multiciliated cells (MCCs) rely on motile cilia, anchored by basal bodies (BBs), for coordinated function.
- Proteins Lrrcc1 and Ccdc61 are known BB components in basal eukaryotes.
Purpose of the Study:
- To investigate the roles of Lrrcc1 and Ccdc61 in vertebrate MCCs.
- To characterize their localization and function within the Xenopus embryonic ciliated epidermis.
Main Methods:
- Gene knockdown experiments in Xenopus tadpoles.
- Immunofluorescence microscopy to assess protein localization.
- Analysis of BB docking, spacing, polarization, and ciliary beating.
Main Results:
- Lrrcc1 and Ccdc61 were confirmed as BB components in Xenopus MCCs, localizing near striated rootlets.
- Depletion of Lrrcc1 or Ccdc61 led to defects in BB organization and apical cytoskeleton.
- Impaired ciliary beating and reduced fluid flow were observed, increasing mortality upon bacterial challenge.
Conclusions:
- Lrrcc1 and Ccdc61 are essential for vertebrate BB biogenesis and function in MCCs.
- Proper BB organization by these proteins is critical for establishing physiological ciliary function.
- This study highlights the evolutionary conservation of BB components and their importance for epithelial function and host defense.
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