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A simple quantitative HPLC assay for ifosfamide in biological fluids
J M Margison1, P M Wilkinson, T Cerny
1Department of Clinical Pharmacology, Christie Hospital, Holt Radium Institute, Manchester, UK.
Biomedical Chromatography : BMC
|June 1, 1986
Summary
A new HPLC method accurately measures Ifosfamide (I) in human serum, crucial for understanding its drug behavior. This validated technique provides reliable pharmacokinetic data for patient treatment.
Area of Science:
- Analytical Chemistry
- Pharmacology
Background:
- Ifosfamide (I) is a critical chemotherapeutic agent.
- Accurate measurement of Ifosfamide in biological samples is essential for pharmacokinetic studies.
Purpose of the Study:
- To develop and validate a high-performance liquid chromatography (HPLC) method for quantifying Ifosfamide in human serum.
- To assess the suitability of the developed HPLC method for pharmacokinetic analyses.
Main Methods:
- Solvent extraction of Ifosfamide from human serum.
- Reverse-phase HPLC with UV detection at 190 nm.
- Construction of standard curves and determination of the limit of detection (100 ng/ml).
Main Results:
- Linear standard curves were obtained for Ifosfamide concentration.
- 14C-labelled Ifosfamide confirmed the method's specificity.
- HPLC and gas chromatography yielded indistinguishable concentration-time profiles.
Conclusions:
- The developed HPLC method is reliable and suitable for determining Ifosfamide pharmacokinetics in biological specimens.
- This method facilitates accurate drug monitoring and dosage adjustments in patients treated with Ifosfamide.