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RTA and LANA Competitively Regulate let-7a/RBPJ Signal to Control KSHV Replication.

Chunhong Di1,2, Guoxia Zheng2, Yunheng Zhang2

  • 1Affiliated Hospital, Hangzhou Normal University, Hangzhou, China.

Frontiers in Microbiology
|January 24, 2022
PubMed
Summary
This summary is machine-generated.

Kaposi's sarcoma-associated herpesvirus (KSHV) replication is controlled by a competition between LANA and RTA proteins for the let-7a/RBPJ pathway. RTA increases RBPJ, while LANA decreases it, impacting KSHV replication.

Keywords:
Kaposi’s sarcoma-associated herpesvirusRBPJlatency-associated nuclear antigenlet-7areplication and transcription activator

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Area of Science:

  • Virology
  • Molecular Biology
  • Gene Regulation

Background:

  • Recombination signal binding protein for immunoglobulin kappa J region (RBPJ) plays a dual role in Kaposi's sarcoma-associated herpesvirus (KSHV) replication.
  • RBPJ interacts with replication and transcription activator (RTA) for lytic replication and latency-associated nuclear antigen (LANA) for latent infection.
  • Previous studies indicated LANA downregulates RBPJ via upregulation of miRNA let-7a.

Purpose of the Study:

  • To investigate whether RTA regulates RBPJ expression.
  • To elucidate the regulatory mechanism of RBPJ by RTA and LANA during KSHV replication.
  • To understand the competition between RTA and LANA for the let-7a/RBPJ signaling pathway.

Main Methods:

  • Analysis of RBPJ expression levels in correlation with RTA and LANA expression during KSHV replication.
  • Quantification of let-7a and RBPJ expression levels.
  • Experimental manipulation of RBPJ levels (knockdown) to assess its impact on RTA and LANA promoter activity.

Main Results:

  • RTA increases RBPJ expression by decreasing let-7a levels.
  • RBPJ expression positively correlates with RTA and negatively with LANA during KSHV replication.
  • Knockdown of RBPJ inhibits RTA and LANA promoter self-activation and weakens LANA's inhibitory effect on the RTA promoter.

Conclusions:

  • RTA and LANA compete for the let-7a/RBPJ signaling pathway to modulate KSHV replication.
  • The regulation of RBPJ expression by RTA and LANA is crucial for controlling KSHV replication dynamics.
  • This study reveals a novel regulatory mechanism involving RTA, LANA, let-7a, and RBPJ in KSHV infection.