Synergistic integration of wastewaters from second generation ethanol plant for algal biofuel production: an industrially relevant option

The present study provides an integrated method for utilizing the wastewaters from second generation (2G) ethanol pretreatment plant for microalgal biomass and lipid production. The study was conducted using a mixture of wastewaters (referred as MW; pH 4.3) generated after washing of acidic and alkaline-soaked lignocellulosic biomass prior to pretreatment process. The growth studies indicated that the thermotolerant strain of Chlorella pyrenoidosa (C. pyrenoidosa) M18 exhibited higher cell proliferation in wastewater as compared to freshwater. About 20-25% enhancement in biomass (509 mg L^-1 d^-1 ± 3.09) and lipid productivity (146 mg L^-1 d^-1 ± 1.34) was observed in MW. The total chlorophyll content and variable fluorescence by maximum fluorescence (Fv/Fm) ratio of strain cultivated in MW were 10.32 µg mL^-1 and 0.75, respectively. The use of MW also enhanced the content of saturated and monounsaturated fatty acids in total lipid. The exhausted wastewater medium obtained after harvesting the auto-flocculated biomass was also reused up to three successive growth cycles. The recycled medium without any nutrient addition could be used for two subsequent rounds with enhanced biomass (520 mg L^-1 d^-1 ± 4.07) and lipid (157.71 mg L^-1 d^-1 ± 1.09) productivities. This synergistic approach of cultivating thermotolerant microalgae with wastewater from 2G pretreatment plant provides an economical setup for development of commercial algal biofuel technology.