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Updated: Oct 5, 2025
Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
Published on: June 9, 2017
The DNA damage repair-related gene PKMYT1 is a potential biomarker in various malignancies
Changjian Shao1,2, Yuanyong Wang1, Minghong Pan1
1Department of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an, China.
Background:
Protein kinase membrane associated tyrosine/threonine 1 (PKMYT1) regulates cell cycle and is a part of DNA damage repair (DDR)-related signaling. Recent studies have identified a role for PKMYT1 in tumor immunity and DDR. Thus, we initiated this study aiming to characterize the molecular and immunological portrait of PKMYT1 in cancer.
Methods:
Transcriptomic data extrapolated from Genotype-Tissue Expression (GTEx), The Cancer Genome Atlas (TCGA), and Cancer Cell Line Encyclopedia (CCLE) datasets were used to determine the mRNA expression levels of PKMYT1. PKMYT1 mRNA expression status was correlated with patients' prognosis as well as immune neoantigens, and immune checkpoints in 34 different tumors. The Tumor Immune Estimation Resource (TIMER) dataset was used to analyze immune infiltrating scores.
Results:
PKMYT1 mRNA is differentially expressed in common tumors and high expression levels of PKMYT1 mRNA is associated with poor prognosis except for malignant thymoma (THYM). In addition, PKMYT1 mRNA expression was correlated with tumor-infiltrating immune cells particularly in lung squamous cell carcinoma, esophageal carcinoma, THYM, and lung adenocarcinoma. An upregulation of immune checkpoints and neoantigens was observed in tumors with a high PKMYT1 mRNA expression. Data from gene set enrichment analysis (GSEA) revealed that PKMYT1 is involved in tumor immunogenicity, metabolism, and cell cycle progression.
Conclusions:
PKMYT1 is differentially expressed in various cancers and exerts an important effect on tumor immunity and progression. The PKMYT1 gene holds the potential as a new potential biomarker. Therefore, further studies are clearly needed to elaborate our findings.
Insights
Protein kinase membrane associated tyrosine/threonine 1 (PKMYT1) is altered in various cancers, impacting tumor immunity and progression. PKMYT1 may serve as a novel biomarker for cancer prognosis and immunotherapy.
Area of Science:
- Oncology
- Cancer Immunology
- Molecular Biology
Background:
- Protein kinase membrane associated tyrosine/threonine 1 (PKMYT1) is a key regulator of cell cycle and DNA damage repair (DDR).
- Emerging evidence highlights PKMYT1's role in tumor immunity and DDR pathways.
- This study investigates the molecular and immunological landscape of PKMYT1 in cancer.
Purpose of the Study:
- To characterize the molecular and immunological profile of PKMYT1 across various cancer types.
- To assess the association of PKMYT1 expression with patient prognosis and immune infiltration.
- To explore the potential of PKMYT1 as a prognostic biomarker in cancer.
Main Methods:
- Utilized transcriptomic data from GTEx, TCGA, and CCLE to analyze PKMYT1 mRNA expression.
- Correlated PKMYT1 expression with patient prognosis, neoantigens, and immune checkpoints in 34 tumors.
- Employed TIMER dataset for immune cell infiltration analysis and GSEA for pathway enrichment.
Main Results:
- PKMYT1 mRNA was differentially expressed in multiple tumors, with high expression linked to poor prognosis (except THYM).
- PKMYT1 expression correlated with immune cell infiltration in lung squamous cell carcinoma, esophageal carcinoma, THYM, and lung adenocarcinoma.
- Upregulation of immune checkpoints and neoantigens observed in high PKMYT1-expressing tumors, with GSEA indicating roles in immunogenicity, metabolism, and cell cycle.
Conclusions:
- PKMYT1 exhibits differential expression in cancers and significantly influences tumor immunity and progression.
- The PKMYT1 gene presents potential as a novel biomarker for cancer prognosis and immunotherapy.
- Further research is warranted to validate these findings and explore therapeutic implications.
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