AlliGator: A Phasor Computational Platform for Fast in vivo Lifetime Analysis
Sez-Jade Chen1, Nattawut Sinsuebphon1, Margarida Barroso2
1Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA.
Abstract:
A fast time-gated phasor analysis tool was developed and used to monitor kinetics of NIR FLIM-FRET in vitro and in vivo. The results were validated by comparison with standard two-component fluorescence decay fitting analysis.
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