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Related Concept Videos

Phasor Arithmetics01:13

Phasor Arithmetics

412
Phasors and their corresponding sinusoids are interrelated, offering unique insights into the behavior of alternating current (AC) circuits. One way to understand this relationship is through the operations of differentiation and integration in both the time and phasor domains.
When the derivative of a sinusoid is taken in the time domain, it transforms into its corresponding phasor multiplied by j-omega (jω) in the phasor domain, where j is the imaginary unit, and ω is the angular...
412
Phasor Relationships for Circuit Elements01:16

Phasor Relationships for Circuit Elements

691
Phasor representation is a powerful tool used to transform the voltage-current relationship for resistors, inductors, and capacitors from the time domain to the frequency domain. This transformation simplifies the analysis of alternating current (AC) circuits.
In the time domain, Ohm's law provides a fundamental relation between the current flowing through a resistor and the voltage across it:
691
Phasors01:12

Phasors

708
Phasors are a powerful mathematical tool used to analyze alternating current (AC) circuits. They provide a complex number representation of sinusoids, with the magnitude of the phasor equating to the amplitude of the sinusoid and the angle of the phasor representing the phase measured from the positive x-axis.
One of the significant benefits of using phasors is that they simplify the analysis of AC circuits by eliminating the time dependence of the current and voltage. This transformation...
708
Kirchoff's Laws using Phasors01:12

Kirchoff's Laws using Phasors

529
Analyzing AC circuits in electrical systems is a fundamental aspect of electrical engineering. In these circuits, AC power is supplied from a distribution panel and wired to various household appliances in parallel. To perform a comprehensive analysis, electrical engineers use Kirchhoff's voltage and current laws, which are equally applicable in AC circuits as in DC circuits.
Kirchhoff's voltage law (KVL) states that the sum of phasor voltages around a closed loop in an AC circuit...
529
Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

789
The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
789

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Related Experiment Video

Updated: Oct 5, 2025

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy
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AlliGator: A Phasor Computational Platform for Fast in vivo Lifetime Analysis.

Sez-Jade Chen1, Nattawut Sinsuebphon1, Margarida Barroso2

  • 1Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA.

Optical Molecular Probes, Imaging and Drug Delivery
|January 24, 2022
PubMed
Summary

A new tool for time-gated phasor analysis enables rapid monitoring of near-infrared fluorescence lifetime imaging microscopy Förster resonance energy transfer (NIR FLIM-FRET) kinetics. This method was validated against traditional fluorescence decay fitting for both in vitro and in vivo applications.

Keywords:
(170.3650) Lifetime-based sensing(170.6920) Time-resolved imaging(260.2510) Fluorescence

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Area of Science:

  • Biophysics
  • Optical Imaging
  • Biochemistry

Background:

  • Fluorescence lifetime imaging microscopy (FLIM) is crucial for studying molecular interactions.
  • Förster resonance energy transfer (FRET) provides insights into proximity and binding events.
  • Accurate kinetic monitoring of FRET is essential for understanding dynamic biological processes.

Purpose of the Study:

  • To develop a rapid time-gated phasor analysis tool for kinetic measurements.
  • To apply this tool for monitoring near-infrared (NIR) FLIM-FRET.
  • To validate the developed tool against established methods.

Main Methods:

  • Development of a novel time-gated phasor analysis algorithm.
  • Application of the tool to monitor NIR FLIM-FRET kinetics.
  • Validation using standard two-component fluorescence decay fitting analysis.

Main Results:

  • Successful development of a fast time-gated phasor analysis tool.
  • Effective monitoring of NIR FLIM-FRET kinetics was achieved.
  • Results were validated, demonstrating accuracy and reliability.

Conclusions:

  • The developed time-gated phasor analysis tool offers a fast and reliable method for kinetic studies.
  • This tool is applicable for both in vitro and in vivo NIR FLIM-FRET measurements.
  • It provides a valuable alternative to conventional kinetic analysis techniques.