Related Experiment Video

Updated: Oct 5, 2025

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.5K

SYT7 plays a role in promoting thyroid cancer by mediating HMGB3 ubiquitination

Shuai Dong1, Jun Pan1, Yi-Bin Shen1

  • 1The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.

Endocrine-Related Cancer
|January 24, 2022
PubMed

Insights

SYT7 and HMGB3 are elevated in thyroid cancer, promoting tumor growth. SYT7 targets HMGB3 via BRCA1, inhibiting its ubiquitination and boosting cancer progression. Targeting SYT7 or HMGB3 may offer new thyroid cancer treatments.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Thyroid cancer is a common endocrine malignancy requiring novel therapeutic targets.
  • Understanding molecular mechanisms driving thyroid cancer progression is crucial for effective treatment strategies.

Purpose of the Study:

  • To investigate the role of SYT7 and its downstream target HMGB3 in thyroid cancer.
  • To elucidate the molecular mechanism by which SYT7 influences thyroid cancer progression.

Main Methods:

  • Immunohistochemical staining, qPCR, and Western blot to assess SYT7 and HMGB3 expression.
  • Cell function assays (proliferation, migration, apoptosis) to evaluate SYT7 and HMGB3 effects.
  • In vivo tumor growth studies and molecular analyses (GeneChip, IPA, UbiBrowser, Co-IP) to determine the SYT7-BRCA1-HMGB3 pathway.

Main Results:

  • SYT7 and HMGB3 expression were significantly upregulated in thyroid cancer tissues and cells.
  • SYT7 knockdown inhibited thyroid cancer cell proliferation and migration while promoting apoptosis; overexpression had opposite effects.
  • SYT7 interacted with BRCA1 to inhibit HMGB3 ubiquitination, leading to increased HMGB3 protein levels and promoting thyroid cancer progression.

Conclusions:

  • SYT7 and HMGB3 are key drivers of thyroid cancer progression.
  • The SYT7/BRCA1/HMGB3 axis represents a potential therapeutic target for thyroid cancer treatment.
Keywords:
HMGB3SYT7apoptosismigrationproliferationthyroid cancer

More Related Videos

SUMO-Binding Entities SUBEs as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer08:29

SUMO-Binding Entities SUBEs as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

Published on: November 1, 2019

7.3K
Induction and Analysis of Epithelial to Mesenchymal Transition10:37

Induction and Analysis of Epithelial to Mesenchymal Transition

Published on: August 27, 2013

36.1K

Related Concept Videos

Synthesis and Regulation of Thyroid Hormones01:20

Synthesis and Regulation of Thyroid Hormones

Low blood levels of the thyroid hormones — triiodothyronine (T3) and thyroxine (T4) — signal the hypothalamus to release the thyrotropin-releasing hormone (TRH). TRH then reaches the pituitary gland and stimulates the release of thyroid-stimulating hormone(TSH) into the bloodstream.
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The...
5.4K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.7K
Functions of Thyroid Hormones01:18

Functions of Thyroid Hormones

The thyroid hormone (TH) plays a pivotal role in the intricate orchestration of physiological processes, exerting profound effects on development, metabolism, and homeostasis throughout different life stages.
TH is indispensable for the normal development and maturation of the skeletal, muscular, and nervous systems during fetal and childhood growth. It facilitates bone mineral turnover and regulates protein synthesis in developing tissues, contributing significantly to overall growth and...
3.4K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.9K
The Thyroid Gland01:23

The Thyroid Gland

The thyroid gland is a small, butterfly-shaped gland located in the neck and covers the anterior surface of the trachea. The gland has two lateral lobes connected by a thin tissue mass called the isthmus. Internally, each lobe comprises many small spherical structures known as thyroid follicles, surrounded by a network of blood vessels.
The follicles have a central cavity lined by simple cuboidal to squamous epithelial cells called follicular cells. These cells produce the glycoprotein...
4.6K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.2K