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Related Concept Videos

Erythropoiesis01:14

Erythropoiesis

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Red blood cells  (RBCs) transport oxygen to all body tissues. These cells survive only for 120 days and then need to be replenished. Erythropoiesis is the process of RBC production. In healthy individuals, erythropoiesis ensures all tissues are amply supplied with oxygen. In addition, blood loss due to injury leads to a drop in the physiological oxygen level that will cause erythropoiesis. Any defect in erythropoiesis leads to several physiological disorders, including thalassemia, anemia,...
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Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
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Related Experiment Video

Updated: Oct 5, 2025

Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells
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RUNX3 overexpression inhibits normal human erythroid development.

Ana Catarina Menezes1, Christabel Dixon1, Anna Scholz1

  • 1Division of Cancer & Genetics, Department of Haematology, School of Medicine, Cardiff University, Cardiff, Wales, CF14 4XN, UK.

Scientific Reports
|January 25, 2022
PubMed
Summary

RUNX3 downregulation is crucial for normal human red blood cell development (erythropoiesis). Suppressing RUNX3 promotes the final stages of red blood cell maturation, offering insights into blood cancers.

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Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
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Area of Science:

  • Hematology
  • Molecular Biology
  • Cell Biology

Background:

  • RUNX proteins are transcription factors vital for cell processes.
  • RUNX3 is implicated in blood formation and blood cancers.

Purpose of the Study:

  • To investigate the role of RUNX3 in normal human erythropoiesis.
  • To understand how RUNX3 expression impacts red blood cell development.

Main Methods:

  • Human CD34+ cells were genetically modified to overexpress or reduce RUNX3.
  • RUNX3 mRNA levels were analyzed during erythropoiesis.
  • Cell growth, differentiation markers (glycophorin A), cell size, and morphology were assessed.

Main Results:

  • RUNX3 levels naturally decrease during erythropoiesis.
  • RUNX3 overexpression modestly affected early erythroid development but promoted late-stage growth.
  • Overexpression inhibited terminal differentiation, leading to larger cells with less mature morphology.

Conclusions:

  • RUNX3 suppression is necessary for normal terminal erythroid differentiation.
  • Downregulation of RUNX3 facilitates the final stages of red blood cell development.
  • Understanding RUNX3's role may advance knowledge of hematological malignancies.