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Updated: Oct 5, 2025
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Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis
Published on: September 7, 2021
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Glutamine as a Potential Noninvasive Biomarker for Human Embryo Selection
Sui-Bing Miao1, Yan-Ru Feng2, Xiao-Dan Wang1
1Institute of Reproductive Medicine of Shijiazhuang, The Fourth Hospital of Shijiazhuang, Gynecology and Obstetrics Hospital Affiliated to Hebei Medical University, 206 East Zhongshan Road, Shijiazhuang, 050011, China.
Reproductive Sciences (Thousand Oaks, Calif.)
|January 25, 2022
Summary
Glutamine consumption is linked to embryo quality and chromosomal abnormalities. Higher glutamine use indicates poorer embryo quality and aneuploidy, suggesting its potential as a biomarker in assisted reproduction.
Area of Science:
- Reproductive biology
- Embryology
- Metabolomics
Background:
- Assisted reproduction treatments aim to improve embryo development.
- Assessing embryo quality and ploidy is crucial for successful implantation.
- Metabolic profiling offers insights into embryo health.
Purpose of the Study:
- To investigate the association between glutamine consumption and embryo quality.
- To determine if glutamine consumption correlates with aneuploidy in human embryos.
Main Methods:
- Retrospective analysis of spent embryo culture media from IVF patients undergoing PGT.
- Quantification of glutamine levels using gas chromatography-mass spectrometry.
- Assessment of embryo quality (cell number, fragmentation) and ploidy status.
Main Results:
- Glutamine consumption significantly differed between good-quality and poor-quality embryos.
- Aneuploid embryos exhibited significantly higher glutamine consumption than euploid embryos.
- Glutamine consumption showed strong predictive value for identifying aneuploidy (AUC=0.938).
Conclusions:
- Glutamine consumption is a significant indicator of embryo quality and aneuploidy.
- Elevated glutamine uptake may represent a compensatory response to oxidative stress.
- Glutamine levels in culture media could serve as a non-invasive biomarker for embryo assessment and aneuploidy screening.
