[Polyamine-producing Bacteria Regulated the Community Structure of Rhizosphere Bacteria and Reduced the Absorption of...

Some functional microorganisms in the soil immobilize heavy metals by adsorption and precipitation, prevent the absorption of heavy metals by crops, and play an important role in the passivation and remediation of medium and mild heavy metal-contaminated soil. A pot experiment was conducted to study the effects of the exogenous polyamine-producing bacterium Bacillus sp. N3 on Cd uptake and the bacterial community composition and function in the rhizosphere soil. The results showed that strain N3 significantly reduced the contents of Cd (64.7%) in wheat grain and DTPA-Cd (50.1%) in rhizosphere soil and increased the pH (from 6.84 to 6.97) and polyamine content. High-throughput sequencing results showed that inoculation of strain N3 reduced the diversity of the bacterial community; however, it increased the relative abundances of β-Proteobacteria, Bacteroidetes, and Firmicutes in wheat rhizosphere soil. Meanwhile, strain N3 also increased the relative abundances of heavy metal-immobilizing bacteria and plant growth-promoting bacteria (Bacillus, Arthrobacter, Brevundimonas, Ensifer, Pedobacter, Rhizobium, Pseudomonas, Enterobacter, and Serratia) in wheat rhizosphere soil. The PICRUSt function prediction showed that strain N3 increased the copy number of genes involved in antioxidant capacity, hormone synthesis, and sulfur metabolism in wheat rhizosphere soil. These results indicated that the polyamine-producing bacteria N3 reduced the DTPA-Cd content by increasing the pH; the polyamine contents; the abundances of bacteria with heavy metal-immobilizing or plant growth-promoting traits; and the metabolic pathway involved in antioxidant capacity, hormone synthesis, and sulfur metabolism in wheat rhizosphere soil, thus inhibiting the absorption of Cd by wheat. The results provide theoretical basis and technical support for restoring farmland with excessive heavy metals and ensuring the safe production of wheat.