Multienzymatic Antioxidant Activity of Manganese-Based Nanoparticles for Protection against Oxidative Cell Damage

Oxidative stress is related to many diseases, but available clinical treatment methods are currently limited. Exploitation of enzyme-mimicking nanomaterials (nanozymes) is a promising way for scavenging reactive oxygen species (ROS) and treatment of ROS-related diseases. Herein, the catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GP) mimicking activities are expressed by MnO₂ nanoparticles (MnO₂^-BSA NPs) coated with BSA. Effective ^•OH removal activity is also expressed by MnO₂^-BSA NPs at neutral pH. Apoptosis inhibition and ROS scavenging capabilities of MnO₂^-BSA NPs are evident on the H₂O₂-exposed BEAS-2B cells line. Western blot analysis indicates that MnO₂^-BSA NPs inhibit H₂O₂-induced apoptosis by mediating the expression of apoptosis-related proteins.