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Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells
Published on: January 21, 2016
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Lysobacter terrestris sp. nov., isolated from soil
1Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Kyonggi-Do 16227, Republic of Korea.
International Journal of Systematic and Evolutionary Microbiology
|January 25, 2022
Summary
A novel bacterium, Lysobacter terrestris sp. nov. strain II4T, was discovered in Korean soil. This yellow-pigmented, aerobic rod expands the known diversity within the Lysobacter genus.
Area of Science:
- Microbiology
- Bacterial Taxonomy
- Genomics
Background:
- The genus Lysobacter comprises aerobic, Gram-negative bacteria known for diverse metabolic capabilities.
- Accurate taxonomic classification is crucial for understanding bacterial roles in ecosystems and potential applications.
Purpose of the Study:
- To characterize a novel bacterial isolate from Korean soil.
- To determine the phylogenetic and taxonomic position of strain II4T within the Lysobacter genus.
- To propose a new species designation based on comprehensive data.
Main Methods:
- Isolation and cultivation of strain II4T from soil.
- Phylogenetic analysis using 16S rRNA gene sequences.
- Chemotaxonomic analyses including respiratory quinones, polar lipids, and fatty acids.
- Genomic analyses including DNA G+C content, average nucleotide identity (ANI), and in silico DNA-DNA hybridization (DDH).
Main Results:
- Strain II4T is a yellow-pigmented, non-motile, aerobic, rod-shaped bacterium that hydrolyzes casein.
- Phylogenetic analysis places strain II4T within the Lysobacter genus, with closest relatives Lysobacter terrae and Lysobacter niabensis (97.88% and 97.82% 16S rRNA similarity, respectively).
- Genomic data (ANI ≤79.6%, in silico DDH 23.7%) and chemotaxonomic profiles distinguish strain II4T from its closest relatives, supporting its status as a new species.
Conclusions:
- Strain II4T represents a novel species within the genus Lysobacter.
- The proposed name for this new species is Lysobacter terrestris sp. nov.
- The type strain is II4T (=KACC 21196T=NBRC 113956T).
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