Race Characterization of Puccinia striiformis f. sp. tritici in the United States from 2013 to 2017

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is an important disease of wheat. In this study, 1,567 isolates collected from the United States from 2013 to 2017 were tested for virulence on 18 wheat Yr single-gene lines to differentiate races. In total, 72 races, including 20 new, were identified, and their frequencies in different years and different epidemiological regions were determined and compared. The 20 new races had low frequencies, and 7 of them each were detected from only one sample and 10 only in a single year. Frequencies of virulence to Yr10, Yr24, and Yr32 were low (<10%); to Yr1, Yr76, YrTr1, and YrSP were moderate (10 to 40%); and to Yr6, Yr7, Yr8, Yr9, Yr17, Yr27, Yr43, Yr44, and Exp2 were high (>70%), although they varied from year to year and from region to region. No virulence was detected to either Yr5 or Yr15, indicating that these genes were still effective against the pathogen in the United States. Based on the virulence data, the diversity of the U.S. P. striiformis f. sp. tritici population was the highest in 2016 and lowest in 2015, and the diversity of the regional population was the highest in region 1 and lowest in region 11. The yearly populations between consecutive years were closer than nonconsecutive years, and the eastern populations were closer to each other than those among the western populations. The findings are useful for understanding the pathogen evolution and for developing resistant cultivars for control of the disease.