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The Effect of 2 Weeks of Naringenin on AQP4, IL-2 and DNA Damage in Brain Ischemia Reperfusion in Rats
M Somuncu, D Dasdelen, S B Baltaci
1Selcuk University, Medical School, Department of Physiology, 42075, Konya, Turkey -
Abstract:
The aim of this study was to determine the effect of different doses of naringenin (NAR) administration for 2 weeks in rats on brain Aquaporin-4 (AQP4), interleukin-2 (IL-2) and 8-Hydroxydeoxyguanosine (8-OhdG) levels in brain ischemia-reperfusion. Experimental groups were formed as follows; 1-Control; 2-Sham Control; 3 Ischemia/ Reperfusion (I/R); 4-Naringenin (Naringenin 50) + I/R; 5-Naringenin (naringenin 100) + I/R. I/R was performed as 1 hour occlusion of the carotid arteries (ischemia) followed by 1 hour reperfusion. Naringenin was supplied for 2 weeks by intraperitoneal. At the end of the experiment, AQP4, IL-2 and 8-OHdG levels were determined in the brain frontal cortex tissue taken from animals killed under anesthesia. AQP4, IL-2 and 8-OHdG levels increased significantly in I/R group. However, both 50 mg/kg and 100 mg/kg two-week administration of naringenin significantly decreased these increased parameters (P 0.001).The results of the study show that intraperitoneal administration of naringenin for two weeks in rats may prevent the damage caused by brain ischemia-reperfusion.
Insights
Naringenin administration for two weeks in rats significantly reduced brain damage markers, including Aquaporin-4 (AQP4), interleukin-2 (IL-2), and 8-Hydroxydeoxyguanosine (8-OhdG), following ischemia-reperfusion injury.
Area of Science:
- Neuroscience
- Pharmacology
- Biochemistry
Background:
- Brain ischemia-reperfusion (I/R) injury is a critical condition leading to significant neurological damage.
- Biomarkers such as Aquaporin-4 (AQP4), interleukin-2 (IL-2), and 8-Hydroxydeoxyguanosine (8-OhdG) are elevated following I/R injury.
- Naringenin, a natural flavonoid, has demonstrated potential therapeutic properties.
Purpose of the Study:
- To investigate the neuroprotective effects of naringenin on brain I/R injury in a rat model.
- To assess the impact of naringenin on specific biomarkers: AQP4, IL-2, and 8-OhdG levels in the brain.
Main Methods:
- Rats were divided into control, sham, I/R, and two naringenin treatment groups (50 mg/kg and 100 mg/kg).
- Naringenin was administered intraperitoneally for two weeks prior to inducing I/R injury (1 hour occlusion, 1 hour reperfusion).
- Brain frontal cortex tissue was analyzed for AQP4, IL-2, and 8-OhdG levels post-experiment.
Main Results:
- Ischemia-reperfusion significantly increased AQP4, IL-2, and 8-OhdG levels in the control group.
- Both 50 mg/kg and 100 mg/kg doses of naringenin significantly reduced these elevated biomarker levels (P < 0.001).
Conclusions:
- Two-week intraperitoneal administration of naringenin demonstrates significant neuroprotective effects against brain ischemia-reperfusion injury in rats.
- Naringenin effectively mitigates the increase in AQP4, IL-2, and 8-OhdG associated with I/R.
- Naringenin shows promise as a therapeutic agent for preventing or treating brain I/R damage.
