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Updated: Oct 5, 2025

Author Spotlight: THP-1 Macrophage Response to LPS/ATP — Unveiling the Pyroptosis, Apoptosis, and Necroptosis Spectrum
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Author Spotlight: THP-1 Macrophage Response to LPS/ATP — Unveiling the Pyroptosis, Apoptosis, and Necroptosis Spectrum

Published on: May 3, 2024

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PP1A prevents ROS-induced pyroptosis by inhibiting MAPK/caspase-3 in mouse adipose tissue

Yizhe Chen1, Meng Che1, Chaowei Li1

  • 1College of Animal Science and Technology, Northwest A&F University, Yangling, China.

The FEBS Journal
|January 26, 2022
PubMed

Insights

The serine/threonine protein phosphatase 1 catalytic subunit α (PP1A) inhibits pyroptosis in mouse adipocytes. PP1A suppresses reactive oxygen species-induced cell death by regulating key signaling pathways and promoting M2 macrophage polarization.

Area of Science:

  • Cell Biology
  • Immunology
  • Metabolism

Background:

  • Pyroptosis is a programmed cell death pathway implicated in cellular development and disease.
  • Recent research indicates pyroptosis occurs in adipocytes, but its regulation remains unclear.
  • The serine/threonine protein phosphatase 1 catalytic subunit α (PP1A) is involved in cell regulation and apoptosis.

Purpose of the Study:

  • To investigate the role and mechanism of PP1A in reactive oxygen species-induced pyroptosis in mouse adipocytes.
  • To elucidate how PP1A influences signaling pathways and macrophage polarization during pyroptosis.

Main Methods:

  • Utilized mouse adipose tissue models.
  • Investigated the effects of PP1A on pyroptosis.
  • Analyzed the reactive oxygen species/mitogen-activated protein kinase/caspase-3 signaling pathway.
  • Assessed M2 macrophage polarization.

Main Results:

  • PP1A was found to suppress pyroptosis in mouse adipocytes.
  • PP1A inhibited the reactive oxygen species/mitogen-activated protein kinase/caspase-3 signaling pathway.
  • PP1A promoted M2 macrophage polarization, suggesting an anti-inflammatory role.

Conclusions:

  • PP1A acts as a negative regulator of pyroptosis in adipocytes under oxidative stress conditions.
  • PP1A's mechanism involves modulating key inflammatory and cell death signaling pathways.
  • These findings offer insights into the therapeutic potential of targeting PP1A in metabolic and inflammatory diseases.
Keywords:
PP1AROSadipose tissuemacrophage polarizationpyroptosis

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