Determination of IC50 values of anticancer drugs on cells by D2O - single cell Raman spectroscopy
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An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
Published on: January 9, 2020
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Wanxue Lv1,2, Boqiang Fu2, Manli Li2
1State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China. luchao@mail.buct.edu.cn.
Chemical Communications (Cambridge, England)
|January 26, 2022
Abstract:
A simple, sensitive and repeatable D2O-single cell Raman spectroscopy method is developed to quantify the inhibitory activity of anticancer drugs on cancer cell metabolism. The IC50 values obtained from A549 cells incubated with cisplatin and taxol are comparable with results of CCK-8 and ATP luminescent cell viability assays.
