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A Deep Mesencephalic Nucleus Circuit Regulates Licking Behavior.

Di Zheng1,2, Jia-Yu Fu1,2, Meng-Yu Tang1,2

  • 1Department of Neurobiology and Department of Neurology of Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310058, China.

Neuroscience Bulletin
|January 26, 2022
PubMed
Summary
This summary is machine-generated.

Researchers discovered a new neural circuit controlling water intake. The central amygdala (CeA) somatostatin-expressing (SST+) neurons regulate deep mesencephalic nucleus (DpMe) activity, influencing licking behavior and promoting hydration.

Keywords:
AmygdalaLickingMidbrainNeural CircuitOptogenetics

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Area of Science:

  • Neuroscience
  • Behavioral Biology
  • Physiology

Background:

  • Licking behavior is crucial for hydration.
  • The deep mesencephalic nucleus (DpMe) is linked to instinctive behaviors, but its role in licking is unclear.
  • Understanding the neural basis of water intake regulation is vital.

Purpose of the Study:

  • To investigate the involvement of the DpMe in licking behavior.
  • To identify the specific neural circuits controlling licking and water intake.
  • To elucidate the functional roles of different neuronal populations in this circuit.

Main Methods:

  • Electrophysiological recordings to monitor DpMe activity during water intake.
  • Optogenetic manipulation of vesicular glutamate transporter 2-positive (VGLUT2+) neurons in the DpMe.
  • Anatomical tracing and immunohistochemistry to identify neuronal connections between the central amygdala (CeA) and DpMe.
  • Optogenetic activation of specific GABAergic neuron populations (SST+ and PKC-δ+) in the CeA projecting to the DpMe.

Main Results:

  • DpMe neuronal activity decreased during water intake.
  • Inhibiting VGLUT2+ neurons in the DpMe led to increased water consumption.
  • Somatostatin-expressing (SST+) GABAergic neurons in the CeA, but not protein kinase C-δ-expressing (PKC-δ+) neurons, innervated DpMe VGLUT2+ neurons.
  • Activation of CeA SST+ GABAergic neurons projecting to the DpMe induced licking behavior and increased water intake.

Conclusions:

  • The DpMe plays a regulatory role in licking behavior, contrary to previous assumptions.
  • A novel neural circuit, CeA(SST+)-DpMe(VGLUT2+), has been identified as a key regulator of licking and water intake.
  • This circuit provides new insights into the neurobiological mechanisms governing hydration and motivated behaviors.