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Related Experiment Video

Updated: Oct 5, 2025

An Optic Nerve Crush Injury Murine Model to Study Retinal Ganglion Cell Survival
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Transcriptome Analyses Reveal Systematic Molecular Pathology After Optic Nerve Crush.

Yuan-Bo Pan1,2, Yiyu Sun2, Hong-Jiang Li3

  • 1Department of Neurosurgery, Southern Medical University Affiliated Fengxian Hospital, Shanghai, China.

Frontiers in Cellular Neuroscience
|January 27, 2022
PubMed
Summary

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Knockdown of Porf-2 restores visual function after optic nerve crush injury.

Cell death & disease·2023

Glial cells

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Regenerative Medicine

Background:

  • Glial cell function in axonal regeneration remains controversial.
  • Previous studies often focus on single genes or cell types, potentially missing broader molecular changes.
  • A comprehensive understanding of glial cell roles in optic nerve injury is needed.

Purpose of the Study:

  • To comprehensively analyze molecular changes in the optic nerve head after injury.
  • To identify key genes and cellular dynamics involved in axonal regeneration.
  • To provide insights into modulating glial cell function for therapeutic purposes.

Main Methods:

  • Performed transcriptome analysis of the optic nerve head over 90 days post-optic nerve crush (ONC).
  • Utilized weighted gene coexpression network analysis (WGCNA) to identify gene modules and hub genes.
Keywords:
WGCNAimmune responsemicrogliaoptic nerve crushtranscriptome

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  • Analyzed glial cell subtype marker changes over time.

    • Main Results:

    • Identified systematic molecular changes in the optic nerve head (ONH) following ONC.
    • Established time-dependent gene module programs and potential therapeutic target hub genes.
    • Revealed that glial cell transitions are time-course dependent.

    Conclusions:

    • This study provides a comprehensive molecular landscape of the optic nerve head after injury.
    • Identified potential therapeutic targets and revealed time-dependent glial cell dynamics.
    • Offers crucial insights for future research aimed at modulating glial function to promote axonal regeneration.