Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

DNA Bacteriophages

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

An ancient genome of Streptococcus pyogenes from a pre-Columbian Bolivian mummy.

Nature communications·2026

Same author

Phage Cocktail Designed for Wastewater Bioremediation Has Limited Effect on Crustacean Filtrator Microbiome Diversity and Health.

Environmental microbiology reports·2026

Same author

Machine learning enables scalable and systematic hierarchical virus taxonomy.

Nature biotechnology·2025

Same author

Changes to virus taxonomy, the international code of virus classification and nomenclature, and the ICTV statutes ratified by the International Committee on Taxonomy of Viruses (2025).

Archives of virology·2025

Same author

A trimodal protein language model enables advanced protein searches.

Nature biotechnology·2025

Same author

Summary of taxonomy changes ratified by the International Committee on Taxonomy of Viruses (ICTV) from the Bacterial Viruses Subcommittee, 2025.

The Journal of general virology·2025

Search research articles

Related Experiment Video

Updated: Oct 5, 2025

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Published on: June 11, 2015

Phage Annotation Guide: Guidelines for Assembly and High-Quality Annotation.

Dann Turner¹, Evelien M Adriaenssens², Igor Tolstoy³

¹Department of Applied Sciences, Faculty of Health and Applied Sciences, University of the West of England, Bristol, United Kingdom.

PHAGE (New Rochelle, N.Y.)

|January 27, 2022

Summary

This summary is machine-generated.

Phage genomics researchers should accurately annotate all bacteriophage (phage) genomes. This guide provides 14 essential questions to address before data submission or publication for new phage genomicists.

Keywords:

annotation guide bacteriophages genome annotation genomics phage taxonomy phages

More Related Videos

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Published on: October 3, 2018

Related Experiment Videos

Last Updated: Oct 5, 2025

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Published on: June 11, 2015

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Published on: October 3, 2018

Area of Science:

Microbiology
Genomics
Bioinformatics

Background:

Bacteriophages (phages) are crucial in microbial ecosystems and phage therapy.
Accurate phage genome annotation is essential for understanding phage biology and applications.
Inconsistent annotation practices can hinder data sharing and interpretation.

Purpose of the Study:

To establish a standardized checklist for phage genome annotation.
To guide researchers new to phage genomics through the annotation process.
To improve the quality and consistency of phage genome submissions.

Main Methods:

Literature review of current phage annotation standards.
Development of a 14-question framework for comprehensive annotation.
Expert consultation within the phage genomics community.

Main Results:

A defined set of 14 critical questions for phage genome annotation.
Guidelines for addressing key aspects of phage genome analysis.
Recommendations for data submission to public repositories.

Conclusions:

Adherence to these questions will enhance phage genome annotation accuracy.
Standardized annotation facilitates robust phage research and data integration.
This framework supports the reliable curation of phage genomic data.