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Integrating Biocatalysis with Viscous Deep Eutectic Solvents in Lab-On-A-Chip Microreactors.

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Deep eutectic solvents (DESs) combined with buffer create low-viscosity media for efficient enzymatic esterifications. Microreactors utilizing these DES-buffer mixtures demonstrate significantly higher productivity than batch reactors for biocatalysis.

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Area of Science:

  • Biocatalysis
  • Green Chemistry
  • Chemical Engineering

Background:

  • Deep eutectic solvents (DESs) offer tunable properties for chemical reactions.
  • Enzymatic esterifications are crucial for synthesizing various esters.
  • Traditional batch reactors can be inefficient for biocatalytic processes.

Purpose of the Study:

  • To investigate the use of low-viscosity DES-buffer mixtures in microreactors for enzymatic esterification.
  • To compare the efficiency of microreactor systems with traditional batch reactors.
  • To explore sustainable and scalable biocatalytic synthesis methods.

Main Methods:

  • Preparation of DES-buffer mixtures (ChCl/glycerol 1:2 with buffer up to 15% v/v) with viscosity below 25 mPa·s at 45°C.
  • Enzymatic esterification reactions conducted in both microreactor and batch reactor setups.
  • Comparison of specific productivities at identical enzyme loadings and 6% conversion.

Main Results:

  • DES-buffer mixtures maintained non-aqueous properties while achieving low viscosity.
  • Microreactors enabled efficient enzymatic esterifications.
  • Microreactor systems achieved significantly higher specific productivities (14 vs. 0.24 mgproduct·min-1·mgbiocat-1) compared to batch reactors.

Conclusions:

  • Low-viscosity DES-buffer media are effective for biocatalysis in microreactors.
  • Microreactor technology offers a substantial improvement in productivity for enzymatic esterifications.
  • Scale-out strategies using DES-water media in microchannels present a promising avenue for industrial synthesis.