The interaction between free Ca²⁺ in host cells and invasion of E. tenella

Eimeria tenella is the most pathogenic and common coccidia that causes chicken coccidiosis. The intracellular free Ca²⁺ of the host cell is closely related to the invasion, development, and proliferation of intracellular parasites. To determine the dynamic changes of intracellular free Ca²⁺ and its function in the process of E. tenella invading host cells, we established a chick embryo cecal epithelial cells model of E. tenella infection. Chick embryo cecal epithelial cells were treated with different Ca²⁺ signal inhibitor, respectively, and then infected with E. tenella. The results showed that extracellular Ca²⁺, Ca²⁺ channels on the cell membrane, IP3R ion channels on the endoplasmic reticulum membrane, and RyR ion channels regulated the free Ca²⁺ in cecal epithelial cells. Through fluorescence labeling and invasion rate detection, we found that the intracellular Ca²⁺ did not change significantly during the invasion of E. tenella, but its stability was critical to the invasion of parasites.