Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

61.7K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
61.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of Alprenolol Hydrochloride as an Anti-prion Compound Using Surface Plasmon Resonance Imaging.

Molecular neurobiology·2018
See all related articles

Related Experiment Video

Updated: Oct 5, 2025

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material
09:50

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material

Published on: September 29, 2017

13.9K

RT-QuIC as ultrasensitive method for prion detection.

Ryuichiro Atarashi1

  • 1Division of Microbiology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan. ryuichiro_atarashi@med.miyazaki-u.ac.jp.

Cell and Tissue Research
|January 27, 2022
PubMed
Summary

Real-time quaking-induced conversion (RT-QuIC) is a highly sensitive prion seeding assay. This method offers advantages over Western blotting for prion disease diagnosis and quantification.

Keywords:
Prion diseasesReal-time quaking-induced conversionRecombinant prion protein

More Related Videos

Prion Safety Laboratory Swipe Test
06:01

Prion Safety Laboratory Swipe Test

Published on: February 14, 2025

885
Assessing Transmissible Spongiform Encephalopathy Species Barriers with an In Vitro Prion Protein Conversion Assay
11:41

Assessing Transmissible Spongiform Encephalopathy Species Barriers with an In Vitro Prion Protein Conversion Assay

Published on: March 10, 2015

7.9K

Related Experiment Videos

Last Updated: Oct 5, 2025

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material
09:50

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material

Published on: September 29, 2017

13.9K
Prion Safety Laboratory Swipe Test
06:01

Prion Safety Laboratory Swipe Test

Published on: February 14, 2025

885
Assessing Transmissible Spongiform Encephalopathy Species Barriers with an In Vitro Prion Protein Conversion Assay
11:41

Assessing Transmissible Spongiform Encephalopathy Species Barriers with an In Vitro Prion Protein Conversion Assay

Published on: March 10, 2015

7.9K

Area of Science:

  • Neuroscience
  • Biochemistry
  • Medical Diagnostics

Background:

  • Prion diseases are fatal neurodegenerative disorders.
  • Detecting abnormal prion protein (PrPSc) is crucial for diagnosis.
  • Current detection methods like Western blotting have limitations in sensitivity.

Purpose of the Study:

  • To highlight the capabilities and applications of Real-time quaking-induced conversion (RT-QuIC) assay.
  • To discuss the advantages of RT-QuIC in prion disease research and diagnostics.
  • To identify future directions for RT-QuIC development and application.

Main Methods:

  • RT-QuIC utilizes recombinant prion protein (recPrP) to amplify PrPSc seeds.
  • The assay measures the formation of abnormal prion protein in real-time.
  • Quantification is achieved by combining RT-QuIC with endpoint dilution.

Main Results:

  • RT-QuIC demonstrates high sensitivity for detecting prion seeding activity.
  • The method is significantly more sensitive than traditional Western blotting.
  • RT-QuIC has been validated for diagnosing sporadic Creutzfeldt-Jakob disease (sCJD).

Conclusions:

  • RT-QuIC is a powerful tool for prion disease diagnosis and research.
  • Its high sensitivity and quantification capabilities make it a valuable diagnostic criterion.
  • Future research should focus on strain differentiation and early diagnostic applications.