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[Fungicidal effect of iodophor preparations on dermatophyte arthrospores]
1Klinika Chorób Zakaźnych Zwierzat AR w Lublinie.
Abstract:
The purpose of the investigations was to determine antifungal activity of iodophores: Pollena-Jod K and Mycofix. In vitro studies 3 strains of Trichophyton mentagrophytes from Sabouraud liquid medium and arthrospores of this fungus in skin scrapings obtained from sick foxes were examined. The in vivo fungicidal effect of the iodophores was studied in foxes and in young bulls, where 5 and 10% solutions of the preparations were used three times. It was found that the exact inactivation of arthrospores is possible when 8-fold or even 10-fold higher concentrations of the preparations were used than that used for inactivation of vegetative cells. After 1 h exposition vegetative cells were destroyed by 0.4% solution of Pollena-Jod K or Mycofix, however, 5% solution of Pollena-Jod K and 3% solution of Mycofix destroyed arthrospores. In the field studies Mycofix revealed higher fungicidal effects. The index of isolation of the fungus from skin scrapings for animals treated with Mycofix was lower by 38% in comparison to that for animals treated with Pollena-Jod K. Despite three times application of 5% and 10% solutions of the examined iodophores on fungal foci in animals, a part of arthrospores survived. For the determination of fungicidal activity of antimycotic drugs used to prevent or control of dermatomycoses, arthrospores present in skin scrapings of sick animals may be used.
Insights
Iodophores Pollena-Jod K and Mycofix show antifungal activity against Trichophyton mentagrophytes. Mycofix demonstrated superior efficacy in field studies for treating animal dermatomycoses.
Area of Science:
- Veterinary Mycology
- Antimicrobial Agents
Background:
- Dermatomycoses are common fungal infections in animals.
- Iodophores are recognized for their antimicrobial properties.
Purpose of the Study:
- To evaluate the in vitro and in vivo antifungal activity of two iodophore preparations, Pollena-Jod K and Mycofix.
- To compare the efficacy of these iodophores against Trichophyton mentagrophytes, a causative agent of animal ringworm.
Main Methods:
- In vitro testing involved exposing Trichophyton mentagrophytes strains and arthrospores to varying concentrations of iodophores.
- In vivo studies utilized iodophore solutions on infected foxes and young bulls.
- Fungal isolation rates from skin scrapings were used to assess treatment effectiveness.
Main Results:
- Both Pollena-Jod K and Mycofix effectively inactivated vegetative fungal cells at low concentrations (0.4%).
- Arthrospores required significantly higher concentrations (5% Pollena-Jod K, 3% Mycofix) for inactivation.
- Mycofix showed a 38% lower fungal isolation rate compared to Pollena-Jod K in field trials.
- Complete eradication of arthrospores was not achieved in vivo with the tested concentrations and application frequency.
Conclusions:
- Iodophores exhibit antifungal activity against Trichophyton mentagrophytes, with varying efficacy against vegetative cells and arthrospores.
- Mycofix presents greater fungicidal effects in vivo for treating animal dermatomycoses.
- Arthrospores in skin scrapings are a relevant model for determining the efficacy of antimycotic drugs.