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NCAPD3 promotes prostate cancer progression by up-regulating EZH2 and MALAT1 through STAT3 and E2F1

Zuolei Jing1, Qianmei Liu1, Wanlin Xie1

  • 1College of Life Sciences, Nanjing Normal University, Nanjing 210023, Jiangsu, China.

Cellular Signalling
|January 27, 2022
PubMed

Insights

Nuclear Cap-Binding Domain Protein 3 (NCAPD3) promotes prostate cancer (PCa) progression. High NCAPD3 expression up-regulates EZH2 and MALAT1, enhancing PCa cell growth.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Cell Biology

Background:

  • Condensin II, a complex regulating chromosome structure, has subunits like NCAPD3.
  • The role of NCAPD3 in prostate cancer (PCa) and its underlying molecular mechanisms remain unclear.
  • Understanding NCAPD3's function is crucial for developing targeted PCa therapies.

Purpose of the Study:

  • To investigate the role of NCAPD3 in prostate cancer (PCa) progression.
  • To elucidate the molecular mechanisms by which NCAPD3 influences PCa development.
  • To determine the relationship between NCAPD3, EZH2, and MALAT1 in PCa.

Main Methods:

  • Analysis of NCAPD3 expression levels in PCa tissues.
  • Investigated the effects of NCAPD3 overexpression and knockdown on PCa cell growth in vitro and in vivo.
  • Examined the molecular interactions involving NCAPD3, STAT3, E2F1, EZH2, and MALAT1 using promoter recruitment assays.

Main Results:

  • NCAPD3 is highly expressed in PCa and promotes tumor growth.
  • High NCAPD3 expression increases STAT3 and E2F1 levels, leading to enhanced EZH2 and MALAT1 expression.
  • Knockdown of NCAPD3 inhibits PCa cell proliferation, while overexpression enhances it.

Conclusions:

  • NCAPD3 acts as a tumor promoter in prostate cancer.
  • NCAPD3 enhances PCa progression by up-regulating EZH2 and MALAT1 expression.
  • Targeting NCAPD3 may represent a novel therapeutic strategy for prostate cancer.
Keywords:
E2F1EZH2MALAT1NCAPD3Prostate cancerSTAT3

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