New NIR spectroscopic probe with a large Stokes shift for Hg²⁺ and Ag⁺ detection and living cells imaging

A new near-infrared (NIR) probe based on a coumarinyl ligand (CL) was designed and synthesized. The probe CL can be used for simultaneous fluorescent turn-on and colorimetric detection of Hg²⁺ and Ag⁺ in ethanol/water medium. Colorless solution of probe CL changed to light yellow or dark yellow after addition of Hg²⁺ or Ag⁺ ions. Meanwhile the maximum absorption band shifted from 379 nm to 404 nm and the intensity increased enormously (for Hg²⁺) or moderately (for Ag⁺). Probe CL displayed an extraordinarily large Stokes shift of 316 nm and addition of Hg²⁺ or Ag⁺ to probe CL induced enhancement in the intensity of fluorescence emission at 695 nm by 15 or 8 fold. The detection limit of CL for Hg²⁺ and Ag⁺ ions is 0.83 and 8.8 μM, respectively. The applicable pH for sensing Hg²⁺ by probe CL is in a broad range of 2-12. Application of probe CL for in vitro U87MG cell imaging to detect Hg²⁺ ions was confirmed.